Inoue Y, Asai Y, Tomita M, Kuribara K, Kobayashi M, Kaneko M, Toba Y
Equine Science Division, Hidaka Training and Research Center, Japan Racing Association, 535-13, Aza-Nishicha, Urakawa-cho, Urakawa- gun, Hokkaido, Japan.
Equine Vet J Suppl. 2006 Aug(36):654-8. doi: 10.1111/j.2042-3306.2006.tb05621.x.
In laboratory animals, man and cell culture experiments, milk basic protein was reported to suppress bone resorption and promote bone formation. However, no studies in horses have previously examined the effect of milk basic protein.
To evaluate the effect of milk basic protein supplementation on bone metabolism in young Thoroughbred horses in training.
Twenty 2-year-old horses in training were used for 90 days in this study. The treatment group was fed a basal diet with 1 g of milk basic protein and the control group a basal diet only. Blood samples were collected on Days 0, 45 and 90 to determine serum calcium (Ca) and biochemical markers of bone metabolism. Radiographs were taken at the start and end of the study to determine radiographic bone aluminium equivalence (RBAE).
Serum osteocalcin (OC) was significantly higher at Day 45 after the beginning of the study in the treatment group compared to that in the control group. The treatment group showed a greater increase in the total RBAE change at the end of this study compared to that in the control group. However, there were no significant differences in serum Ca and carboxy-terminal telopeptide of type I collagen (ICTP) between groups.
These findings provide preliminary evidence that milk basic protein has an effect on bone formation in 2-year-old Thoroughbred horses in training. However, further studies in larger groups of horses are now required to substantiate our findings.
在实验动物、人体及细胞培养实验中，据报道乳碱性蛋白可抑制骨吸收并促进骨形成。然而，此前尚无针对马匹的研究探讨过乳碱性蛋白的作用效果。
评估补充乳碱性蛋白对训练中的年轻纯种马骨代谢的影响。
本研究选用20匹2岁的训练用马，为期90天。治疗组喂食含1克乳碱性蛋白的基础日粮，对照组仅喂食基础日粮。在第0天、第45天和第90天采集血样，以测定血清钙（Ca）及骨代谢的生化标志物。在研究开始和结束时拍摄X光片，以测定X光片骨铝当量（RBAE）。
与对照组相比，治疗组在研究开始后第45天时血清骨钙素（OC）显著更高。与对照组相比，治疗组在本研究结束时总RBAE变化的增幅更大。然而，两组之间血清Ca及I型胶原羧基末端肽（ICTP）并无显著差异。
这些发现提供了初步证据，表明乳碱性蛋白对训练中的2岁纯种马的骨形成有影响。然而，现在需要在更大数量的马匹群体中开展进一步研究，以证实我们的发现。
