不同培养参数对 Muticus 天仙子悬浮培养物生长及生物碱产生的影响
Growth and alkaloid production in suspension cultures of Hyoscyamus muticus as influenced by various cultural parameters.
作者信息
Koul S, Ahuja A, Grewal S
机构信息
Regional Research Laboratory, Jammu Tawi, India.
出版信息
Planta Med. 1983 Jan;47(1):11-6. doi: 10.1055/s-2007-969939.
Suspension cultures of Hyoscyamus muticus L. were raised in RT medium supplemented with NAA 10 (-5)M. Effects of pH, vitamins, sucrose, sodium and potassium succinate, chemical precursors and phytohormones on growth, morphogenesis and total tropane alkaloid production have been examined. Of these, low pH, sodium succinate, precursors and IAA enhanced alkaloid production. Alkaloid synthesis was higher during stationary phase and was found to be independent of morphogenesis.
在添加了10^(-5)M萘乙酸（NAA）的RT培养基中培养了天仙子（Hyoscyamus muticus L.）的悬浮培养物。研究了pH值、维生素、蔗糖、琥珀酸钠和琥珀酸钾、化学前体和植物激素对生长、形态发生和总托烷生物碱产量的影响。其中，低pH值、琥珀酸钠、前体和吲哚乙酸（IAA）提高了生物碱产量。生物碱合成在稳定期较高，且发现其与形态发生无关。
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