Noviello Silvana, Ianniello Filomena, Leone Sebastiano, Esposito Silvano
Dipartimento di Malatte Infettive, Seconda Università degli Studi di Napoli, Italy.
Infez Med. 2006 Mar;14(1):24-8.
The present investigation aimed to compare the in vitro antimicrobial activity of prulifloxacin to that of levofloxacin and ciprofloxacin against recently urinary pathogens. From our large bacterial collection, 978 non-duplicated clinical isolates, derived from patients urinary infections, maintained with minimal sub-culture deep frozen at -70 degrees C in a matrix of Brain Heart Infusion broth +20 % glycerol were tested to determine MICs of prulifloxacin, levolfoxacin and ciprofloxacin by means of broth microdilution technique. For Gram negartive bacteria, no significant diffrences was observed between all three antibiotics. Enterobacteria showed a resistance rate ranging between 6% (Enterobacter spp) and approximately 20% (Escherichia coli). Antibiotic resistance of Pseudomas spp was higher (30.3%). For Gram positive, all methicillin-susceptible Stalphylococcus aureus strains were sensitive whereas 30% of methicillin-resistant and enterococci were resistant to all three antibiotics. Purification exerts its antibacterial activity at lower concentrations than ciprofloxacin and levofloxacin. The in vitro activity of prulifloxacin against urinary pathogens and its high and prolonged urinary concentration following a single oral dose, suggest that for treatment of urinary prulifloxacin is an alternative to other flouroquinolones for treatment of urinary tract infections.
本研究旨在比较普鲁利沙星与左氧氟沙星和环丙沙星对近期泌尿系统病原体的体外抗菌活性。从我们大量的细菌样本中，选取了978株非重复的临床分离株，这些分离株来自患者的泌尿系统感染，保存在含有脑心浸液肉汤+20%甘油的基质中，于-70℃极少传代冻存，采用肉汤微量稀释技术测定普鲁利沙星、左氧氟沙星和环丙沙星的最低抑菌浓度（MIC）。对于革兰氏阴性菌，三种抗生素之间未观察到显著差异。肠杆菌的耐药率在6%（肠杆菌属）至约20%（大肠杆菌）之间。铜绿假单胞菌的抗生素耐药性较高（30.3%）。对于革兰氏阳性菌，所有对甲氧西林敏感的金黄色葡萄球菌菌株均敏感，而30%的耐甲氧西林金黄色葡萄球菌和肠球菌对这三种抗生素均耐药。普鲁利沙星在比环丙沙星和左氧氟沙星更低的浓度下发挥其抗菌活性。普鲁利沙星对泌尿系统病原体的体外活性及其单次口服后在尿液中的高浓度和长时间存在，表明在治疗泌尿系统感染方面，普鲁利沙星是其他氟喹诺酮类药物的替代选择。
