Rutherford M S, Schook L B
Department of Animal Sciences, University of Illinois, Urbana-Champaign.
J Leukoc Biol. 1992 Jan;51(1):69-76. doi: 10.1002/jlb.51.1.69.
Macrophages derived in vitro from bone marrow progenitors (bone marrow-derived macrophages, BMDMs) using either macrophage colony-stimulating factor (CSF-1) or granulocyte-macrophage colony-stimulating factor (GM-CSF) as the myelopoietic stimulus display differential functional, morphological, and mRNA phenotypes. The data presented here demonstrate further that CSF-1- and GM-CSF-derived BMDMs differ in immunologic capacity. GM-CSF-derived BMDMs, when compared to CSF-1-derived BMDMs, showed greater cytolytic activity against tumor necrosis factor alpha (TNF-alpha)-resistant, but not TNF-alpha-sensitive, tumor targets. In contrast, CSF-1-derived BMDMs produced nitrite in response to lipopolysaccharide (LPS) alone, whereas GM-CSF-derived BMDMs required interferon gamma plus LPS treatment. The two BMDM populations also showed differential sensitivities to LPS for secretion of TNF-alpha and nitrite, but the maximal inducible amounts of these factors and prostaglandin E2 were similar between the BMDM populations. Lastly, GM-CSF-derived but not CSF-1-derived BMDMs showed an L-arginine-dependent listeriacidal activity. These results show that the functional heterogeneity of CSF-1- and GM-CSF-derived macrophages is limited and appears to result largely from differences in the activational signals required by each BMDM population to elicit a given function.
使用巨噬细胞集落刺激因子（CSF-1）或粒细胞-巨噬细胞集落刺激因子（GM-CSF）作为骨髓生成刺激物，从骨髓祖细胞体外衍生的巨噬细胞（骨髓来源的巨噬细胞，BMDM）表现出不同的功能、形态和mRNA表型。此处呈现的数据进一步证明，CSF-1衍生的BMDM和GM-CSF衍生的BMDM在免疫能力方面存在差异。与CSF-1衍生的BMDM相比，GM-CSF衍生的BMDM对肿瘤坏死因子α（TNF-α）抗性而非TNF-α敏感的肿瘤靶标表现出更大的细胞溶解活性。相反，CSF-1衍生的BMDM仅对脂多糖（LPS）产生亚硝酸盐，而GM-CSF衍生的BMDM需要干扰素γ加LPS处理。这两种BMDM群体对LPS分泌TNF-α和亚硝酸盐也表现出不同的敏感性，但这些因子和前列腺素E2的最大诱导量在BMDM群体之间相似。最后，GM-CSF衍生而非CSF-1衍生的BMDM表现出L-精氨酸依赖性杀李斯特菌活性。这些结果表明，CSF-1衍生和GM-CSF衍生的巨噬细胞的功能异质性是有限的，并且似乎主要源于每个BMDM群体引发给定功能所需的激活信号的差异。
