Janakiraman Vasantharajan, Forrest William F, Seshagiri Somasekar
Department of Molecular Biology, Genentech Inc., 1 DNA way, South San Francisco, California 94080, USA.
Nat Protoc. 2006;1(5):2271-6. doi: 10.1038/nprot.2006.387.
In this paper, a simple and rapid protocol for determination of baculovirus titers based on increasing viable insect cell size/diameter following virus infection is presented. There are different methods available for determining virus titers such as plaque assays end-point dilution, quantitative real-time polymerase chain reaction and flow cytometry. However, most of these methods are time consuming and labor intensive. The titer estimation method presented here can be completed in approximately 28 h from start to finish. In this method, the Vi-CELL (Beckman Coulter) was used to measure cell diameter change over a range of virus dilutions, following infection. The cell diameter change data were used to compute the virus titer using a statistical method called the method of moments that we have described previously.
本文介绍了一种基于病毒感染后昆虫活细胞大小/直径增加来测定杆状病毒滴度的简单快速方法。有多种方法可用于测定病毒滴度，如噬斑测定、终点稀释、定量实时聚合酶链反应和流式细胞术。然而，这些方法大多耗时且 labor intensive。此处介绍的滴度估算方法从开始到结束大约28小时即可完成。在该方法中，使用Vi-CELL（贝克曼库尔特公司）测量感染后一系列病毒稀释液中细胞直径的变化。利用细胞直径变化数据，通过我们之前描述的一种称为矩量法的统计方法来计算病毒滴度。
