Du Liming, Jiao Fangchan, Chu Jun, Jin Gulei, Chen Ming, Wu Ping
The Key State Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zijingang Campus, Hangzhou 310058, People's Republic of China.
Genomics. 2007 Jun;89(6):697-707. doi: 10.1016/j.ygeno.2007.02.001. Epub 2007 Apr 6.
In this report we define the genes of two-component regulatory systems in rice through a comprehensive computational analysis of rice (Oryza sativa L.) genome sequence databases. Thirty-seven genes were identified, including 5 HKs (cytokinin-response histidine protein kinase) (OsHK1-4, OsHKL1), 5 HPs (histidine phosphotransfer proteins) (OsHP1-5), 15 type-A RRs (response regulators) (OsRR1-15), 7 type B RR genes (OsRR16-22), and 5 predicted pseudo-response regulators (OsPRR1-5). Protein motif organization, gene structure, phylogenetic analysis, chromosomal location, and comparative analysis between rice, maize, and Arabidopsis are described. Full-length cDNA clones of each gene were isolated from rice. Heterologous expression of each of the OsHKs in yeast mutants conferred histidine kinase function in a cytokinin-dependent manner. Nonconserved regions of individual cDNAs were used as probes in expression profiling experiments. This work provides a foundation for future functional dissection of the rice cytokinin two-component signaling pathway.
在本报告中，我们通过对水稻（Oryza sativa L.）基因组序列数据库进行全面的计算分析，确定了水稻中双组分调节系统的基因。共鉴定出37个基因，包括5个组氨酸激酶（细胞分裂素响应组氨酸蛋白激酶）（OsHK1 - 4、OsHKL1）、5个组氨酸磷酸转移蛋白（OsHP1 - 5）、15个A型响应调节因子（OsRR1 - 15）、7个B型RR基因（OsRR16 - 22）以及5个预测的假响应调节因子（OsPRR1 - 5）。描述了蛋白质基序组织、基因结构、系统发育分析、染色体定位以及水稻、玉米和拟南芥之间的比较分析。从水稻中分离出每个基因的全长cDNA克隆。在酵母突变体中对每个OsHK进行异源表达，以细胞分裂素依赖的方式赋予组氨酸激酶功能。各个cDNA的非保守区域用作表达谱实验中的探针。这项工作为未来对水稻细胞分裂素双组分信号通路进行功能剖析奠定了基础。
