Nisbet D R, Pattanawong S, Ritchie N E, Shen W, Finkelstein D I, Horne M K, Forsythe J S
CRC for Polymers, Department of Materials Engineering, Monash University, PO Box 69M, Victoria 3800, Australia.
J Neural Eng. 2007 Jun;4(2):35-41. doi: 10.1088/1741-2560/4/2/004. Epub 2007 Jan 31.
The interaction of murine embryonic cortical neurons on randomly orientated electrospun scaffolds of poly(L-lactide) (P(L)LA) and poly(lactide-co-glycolide) (PLGA) is investigated in this study. The scaffolds were surface treated with different concentrations of KOH to partially hydrolyze the surface and therefore change the surface tension. Hydrophilicity did not significantly influence the number of primary and secondary branches; however, it had a considerable effect on neurite extension. For scaffolds with surface tensions of 40-47 dyn cm(-1) there was a significantly greater overall neurite length for both the primary and secondary branches compared with more hydrophilic scaffolds. Another major finding of this work was that the interfibre distance influenced how the neurites extended. When the interfibre distance was greater than approximately 15 microm the neurites followed the fibres and avoided regions of very high fibre density. At interfibre distances less than approximately 15 microm, the neurites traversed between the fibres. Therefore, this study provided little evidence that contact guidance was the dominating cue in directing neurite extension, instead inferring that chemical cues, possibly from adjacent neurons had induced directional change.
本研究调查了小鼠胚胎皮质神经元与聚（L-丙交酯）（P（L）LA）和聚（丙交酯-共-乙交酯）（PLGA）随机取向的电纺支架之间的相互作用。对支架进行不同浓度的KOH表面处理，以部分水解表面，从而改变表面张力。亲水性对一级和二级分支的数量没有显著影响；然而，它对神经突的延伸有相当大的影响。与亲水性更强的支架相比，表面张力为40-47 dyn cm(-1)的支架，其一级和二级分支的总神经突长度明显更长。这项工作的另一个主要发现是，纤维间距离影响神经突的延伸方式。当纤维间距离大于约15微米时，神经突沿着纤维生长，并避开纤维密度非常高的区域。当纤维间距离小于约15微米时，神经突在纤维之间穿过。因此，本研究几乎没有证据表明接触导向是指导神经突延伸的主要线索，相反推断可能来自相邻神经元的化学线索诱导了方向改变。
