Zhao Yunli, Wang Xiaoying, Zhao Yingchun, Gao Xiaoxia, Bi Kaishun, Yu Zhiguo
School of Pharmacy, Shenyang Pharmaceutical University, P.R. China.
Biol Pharm Bull. 2007 Apr;30(4):617-20. doi: 10.1248/bpb.30.617.
Homoeriodictyol-7-O-beta-D-glucopyranoside (HEDT-Glu) was isolated from Viscum coloratum and identified by MS, 1H- and 13C-NMR. A HPLC method was developed for determination of HEDT-Glu in rat plasma and tissues. All biological samples were pretreated by protein precipitation with acetone. Vanillin was selected as internal standard. The mobile phase consisted of methanol-water-glacial acetic acid (45 : 55 : 0.5, v/v/v). Good linearity were observed over the concentration ranges of 0.1-200.0 microg.ml(-1) in rat plasma and 0.05-5.0 microg.ml(-1) in tissues. Both intra- and inter-day precisions of HEDT-Glu, expressed as the relative standard deviation, were less than 13.1%. Accuracy, expressed as the relative error, ranged from -0.8 to 5.4% in plasma and from -5.6 to 9.4% in tissues. The mean extraction recovery of HEDT-Glu was above 73.17% in biological samples. The described assay method was successfully applied to the pre-clinical pharmacokinetic study of HEDT-Glu. After intravenous administration of HEDT-Glu to rat, AUC and CL(tot) were 16.04+/-3.19 microg.h.ml(-1) and 0.85+/-0.17 l.kg(-1).h(-1), respectively. T(1/2,alpha) and t(1/2,beta) were 0.06+/-0.01 h and 1.27+/-0.31 h, respectively. HEDT-Glu was cleared from the blood and mainly distributed to the liver and small intestine.
从槲寄生中分离得到高圣草素-7-O-β-D-吡喃葡萄糖苷（HEDT-Glu），并通过质谱、1H-和13C-核磁共振进行鉴定。建立了一种高效液相色谱法用于测定大鼠血浆和组织中的HEDT-Glu。所有生物样品均采用丙酮沉淀蛋白进行预处理。选择香草醛作为内标。流动相由甲醇-水-冰醋酸（45 : 55 : 0.5，v/v/v）组成。在大鼠血浆浓度范围为0.1 - 200.0 μg·ml-1和组织浓度范围为0.05 - 5.0 μg·ml-1内观察到良好的线性关系。HEDT-Glu的日内和日间精密度以相对标准偏差表示，均小于13.1%。准确度以相对误差表示，在血浆中为-0.8%至5.4%，在组织中为-5.6%至9.4%。生物样品中HEDT-Glu的平均提取回收率高于73.17%。所描述的分析方法成功应用于HEDT-Glu的临床前药代动力学研究。大鼠静脉注射HEDT-Glu后，AUC和CL(tot)分别为16.04±3.19 μg·h·ml-1和0.85±0.17 l·kg-1·h-1。T(1/2,α)和t(1/2,β)分别为0.06±0.01 h和1.27±0.31 h。HEDT-Glu从血液中清除，主要分布到肝脏和小肠。
