Onal Armağan, Cağlar Sena
Istanbul University, Faculty of Pharmacy, Department of Analytical Chemistry, Istanbul, Turkey.
Chem Pharm Bull (Tokyo). 2007 Apr;55(4):629-31. doi: 10.1248/cpb.55.629.
Simple and reproducible spectrophotometric methods have been developed for determination of dopaminergic drugs used for Parkinson's disease, cabergoline (CAB) and ropinirole hydrochloride (ROP), in pharmaceutical preparations. The methods are based on the reactions between the studied drug substances and ion-pair agents [methyl orange (MO), bromocresol green (BCG) and bromophenol blue (BPB)] producing yellow colored ion-pair complexes in acidic buffers, after extracting in dichloromethane, which are spectrophotometrically determined at the appropriate wavelength of ion-pair complexes. Beer's law was obeyed within the concentration range from 1.0 to 35 microg ml(-1). The developed methods were applied successfully for the determination of these drugs in tablets.
已开发出简单且可重复的分光光度法，用于测定用于治疗帕金森病的多巴胺能药物卡麦角林（CAB）和盐酸罗匹尼罗（ROP）在药物制剂中的含量。这些方法基于所研究的药物物质与离子对试剂[甲基橙（MO）、溴甲酚绿（BCG）和溴酚蓝（BPB）]之间的反应，在酸性缓冲液中反应生成黄色离子对络合物，经二氯甲烷萃取后，在离子对络合物的适当波长下进行分光光度测定。在1.0至35 μg ml⁻¹的浓度范围内符合比尔定律。所开发的方法已成功应用于片剂中这些药物的测定。
