活化白细胞黏附分子（CD166）是早期小鼠心肌细胞的一种表面标志物。

ALCAM (CD166) is a surface marker for early murine cardiomyocytes.

作者信息

Hirata Hirokazu, Murakami Yoshinobu, Miyamoto Yoshiaki, Tosaka Mako, Inoue Kayoko, Nagahashi Ayako, Jakt Lars Martin, Asahara Takayuki, Iwata Hiroo, Sawa Yoshiki, Kawamata Shin

机构信息

Foundation of Biomedical Research and Innovation, Kobe, Japan.

出版信息

Cells Tissues Organs. 2006;184(3-4):172-80. doi: 10.1159/000099624.

DOI:10.1159/000099624

PMID:17409743

Abstract

ALCAM (activated leukocyte cell adhesion molecule, CD166) belongs to the immunoglobulin superfamily and is involved in axon guidance, hematopoiesis, immune response and tumor metastasis. During embryogenesis, mRNA encoding ALCAM was expressed in the cardiac crescent and the neural groove at embryonic day (E) 7.75 and predominately in the tubular heart at E8.5. A newly generated monoclonal antibody against the ALCAM molecule (ALC-48) exclusively stained cardiomyocytes at E8.25-10.5. However, ALCAM expression was lost by cardiomyocytes by E12.5 and its expression shifts to a variety of organs during later stages. ALCAM was found to be a prominent surface marker for cardiomyocytes in early embryonic hearts. The transient expression of ALCAM during early developmental stages marks specific developmental stages in cardiomyocyte differentiation.

摘要

激活白细胞细胞黏附分子（ALCAM，CD166）属于免疫球蛋白超家族，参与轴突导向、造血、免疫反应和肿瘤转移。在胚胎发育过程中，编码ALCAM的mRNA在胚胎第（E）7.75天的心脏新月和神经沟中表达，在E8.5时主要在管状心脏中表达。一种新产生的针对ALCAM分子的单克隆抗体（ALC-48）在E8.25 - 10.5时仅对心肌细胞进行染色。然而，到E12.5时心肌细胞失去了ALCAM表达，在后期其表达转移到多种器官。发现ALCAM是早期胚胎心脏中心肌细胞的一个重要表面标志物。ALCAM在早期发育阶段的短暂表达标志着心肌细胞分化的特定发育阶段。