Sutherland J C, Low H
Proc Natl Acad Sci U S A. 1976 Feb;73(2):276-80. doi: 10.1073/pnas.73.2.276.
We have modified a spectrometer for the measurement of fluorescence-detected magnetic (and natural) circular dichroism (FDMCD). The instrument can be operated either in a direct mode in which the average and polarization-induced differential fluorescent intensities are recorded separately or in a mode which records their ratio. We have measured the FDMCD of tryptophan, which is itself fluorescent. With the aid of an added fluorescent molecule we also measured the FDMCD of Fe(III) cytochrome c, which is nonfluorescent. In both cases, the FDMCD agreed with the conventional transmission-detected magnetic circular dichroism within experimental uncertainty. The FDMCD of both fluorescent and nonfluorescent molecules is potentially a useful technique for investigating optically dense materials and biological molecules in their native environments and systems in which there is inter- or intramolecular energy transfer.
我们改装了一台用于测量荧光检测磁（和自然）圆二色性（FDMCD）的光谱仪。该仪器既可以在直接模式下运行，分别记录平均荧光强度和偏振诱导的差分荧光强度，也可以在记录两者比值的模式下运行。我们测量了色氨酸本身的FDMCD，色氨酸是有荧光的。借助添加的荧光分子，我们还测量了非荧光的Fe(III)细胞色素c的FDMCD。在这两种情况下，FDMCD在实验误差范围内与传统的透射检测磁圆二色性一致。对于荧光和非荧光分子，FDMCD都可能是一种有用的技术，可用于研究光学致密材料以及处于天然环境中的生物分子，以及存在分子间或分子内能量转移的系统。
