Coym Jason W, Roe Brian W
Department of Chemistry, The University of South Alabama, Mobile, AL 36688-0002, USA.
J Chromatogr A. 2007 Jun 22;1154(1-2):182-8. doi: 10.1016/j.chroma.2007.03.075. Epub 2007 Mar 28.
The effect of mobile phase modifier and temperature on gradient reequilibration is examined using three different stationary phases. The stationary phases studied are a traditional C18 phase, a polar endcapped C18 phase, and an alkyl phase with a polar embedded group. It was observed that both temperature and choice of mobile phase organic modifier had an effect on gradient reequilibration volume on both the traditional C18 stationary phase and the polar endcapped phase. On both these phases, at any given temperature, the reequilibration volume was generally smaller when methanol was used as the mobile phase modifier as compared to acetonitrile. As the temperature is increased from 10 to 50 degrees C, significant reductions in reequilibration volume were observed with both mobile phase modifiers. In contrast, neither temperature nor choice of modifier appeared to have much effect on reequilibration volume when the polar embedded group stationary phase was considered.
使用三种不同的固定相研究了流动相改性剂和温度对梯度重新平衡的影响。所研究的固定相分别是传统的C18相、极性封端的C18相和带有极性嵌入基团的烷基相。结果发现，温度和流动相有机改性剂的选择对传统C18固定相和极性封端相的梯度重新平衡体积均有影响。在这两种固定相上，在任何给定温度下，与乙腈相比，当使用甲醇作为流动相改性剂时，重新平衡体积通常较小。随着温度从10℃升高到50℃，两种流动相改性剂的重新平衡体积均显著降低。相比之下，当考虑极性嵌入基团固定相时，温度和改性剂的选择似乎对重新平衡体积均没有太大影响。
