甲状腺中的磷蛋白磷酸酶活性。

Phosphoprotein phosphatase activity in the thyroid.

作者信息

Spaulding S W, Burrow G N

出版信息

Proc Soc Exp Biol Med. 1975 Dec;150(3):568-70. doi: 10.3181/00379727-150-39080.

Abstract

Phosphoprotein phosphatase activity in the calf thyroid was found in various subcellular fractions. The relative amount in each fraction varied according to the substrate used: The 500g fraction had the highest specific activity when protamine was used, while the 5000g fraction was highest when histone was used. Triton X-100 tended to increase activity in all the particulate fractions, the greatest change being found in the 105,000g pellet. DEAE chromatography of the 105,000 g supernatant resolved at least three peaks of phosphoprotein phosphatase activity.

摘要

在小牛甲状腺的各种亚细胞组分中发现了磷蛋白磷酸酶活性。每个组分中的相对含量根据所使用的底物而有所不同：当使用鱼精蛋白时，500g组分具有最高的比活性，而当使用组蛋白时，5000g组分的比活性最高。Triton X-100倾向于增加所有颗粒组分中的活性，在105,000g沉淀中发现的变化最大。对105,000g上清液进行DEAE色谱分析，分离出至少三个磷蛋白磷酸酶活性峰。