Rees Sandra M, Camm Emily J, Loeliger Michelle, Cain Sarah, Dieni Sandra, McCurnin Donald, Shaul Philip W, Yoder Bradley, McLean Catriona, Inder Terrie E
Department of Anatomy and Cell Biology, University of Melbourne, Parkville, Victoria, 3010, Australia.
Pediatr Res. 2007 May;61(5 Pt 1):552-8. doi: 10.1203/pdr.0b013e318045be20.
Inhaled nitric oxide (iNO) enhances ventilation in very preterm infants, but the effects on the brain remain uncertain. We evaluated the impact of iNO on brain growth and cerebral injury in a premature baboon model. Baboons were delivered at 125 d of gestation (term 185 d of gestation) and ventilated for 14 d with either positive pressure ventilation (PPV) (n = 7) or PPV + iNO (n = 8). Brains were assessed histologically for parameters of development and injury. Compared with gestational controls (n = 7), brain and body weights were reduced but brain-to-body weight ratios were increased in all prematurely delivered (PD) animals; the surface folding index (SFI), was reduced in PPV but not PPV + iNO animals. Compared with controls, the brain damage index was increased (p < 0.05) in both cohorts of PD animals. There was no difference between ventilatory regimens, however, in 25% of animals with iNO therapy, there were organized hematomas in the subarachnoid space. Overall, iNO did not alter the extent of brain damage but did result in the presence of hematomas. These results do not confirm any protective or major injurious effect of nitric oxide therapy on the developing brain.
吸入一氧化氮（iNO）可增强极早产儿的通气功能，但对大脑的影响仍不确定。我们在早产狒狒模型中评估了iNO对脑生长和脑损伤的影响。狒狒在妊娠125天（足月为妊娠185天）时分娩，并采用正压通气（PPV）（n = 7）或PPV + iNO（n = 8）进行通气14天。对大脑进行组织学评估，以确定发育和损伤参数。与妊娠对照组（n = 7）相比，所有早产（PD）动物的脑和体重均降低，但脑体重比增加；PPV组动物的表面折叠指数（SFI）降低，但PPV + iNO组动物未降低。与对照组相比，两组PD动物的脑损伤指数均升高（p < 0.05）。然而，通气方案之间没有差异，不过，在接受iNO治疗的动物中，25%出现了蛛网膜下腔有组织的血肿。总体而言，iNO并未改变脑损伤的程度，但确实导致了血肿的出现。这些结果并未证实一氧化氮治疗对发育中的大脑有任何保护作用或重大损伤作用。
