半透明带分析（HZA）作为一种实验模型，用于评估分离的透明带蛋白对精子与小鼠透明带结合的抑制作用。

The hemizona assay (HZA) as an experimental model to evaluate the inhibition of sperm binding to the murine zona pellucida by isolated zona pellucida protein.

作者信息

Windt M L, Franken D R, de Beer P M, Bouic P J, Kruger T F

机构信息

Department of Obstetrics and Gynaecology, University of Stellenbosch, Republic of South Africa.

出版信息

Andrologia. 1991 May-Jun;23(3):209-12. doi: 10.1111/j.1439-0272.1991.tb02539.x.

DOI:10.1111/j.1439-0272.1991.tb02539.x

PMID:1741484

Abstract

Compelling evidence has demonstrated that zona binding represents gamete recognition by sperm binding with high affinity and specificity to complex glycoproteins of the zona pellucida. In the present study we evaluated the hemizona assay (HZA) in the investigation of the interaction of mouse spermatozoa with unfertilized murine oocytes and hemizonae after exposure to solubilized murine zonae pellucidae proteins. The zonae pellucidae were isolated from ovarian tissue following described mincing techniques. The sperm binding characteristics of murine spermatozoa were studied by using SDS-PAGE isolated ZP2 (+/- 120 Kd) and ZP3 (+/- 83 Kd) components of the zona pellucida. Sperm receptor activity was examined in a competitive gamete binding fashion using the HZA as an indicator of sperm/zona interaction. The results illustrated that isolated, solubilized ZP2 and ZP3 glycoprotein moieties of the zona pellucida inhibited sperm binding to hemizonae and oocytes when compared to controls, and that the HZA can be utilized as an internally controlled homologous bioassay to evaluate the effects of zona pellucida proteins on tight binding of spermatozoa to the zona pellucida.

摘要

有力的证据表明，透明带结合代表精子通过与透明带的复合糖蛋白高亲和力和特异性结合来识别配子。在本研究中，我们评估了半透明带试验（HZA），以研究小鼠精子在暴露于可溶性小鼠透明带蛋白后与未受精的小鼠卵母细胞和半透明带的相互作用。按照所述切碎技术从卵巢组织中分离透明带。通过使用SDS-PAGE分离的透明带的ZP2（约120 Kd）和ZP3（约83 Kd）成分研究小鼠精子的精子结合特性。以竞争配子结合方式使用HZA作为精子/透明带相互作用的指标来检查精子受体活性。结果表明，与对照相比，分离的、可溶的透明带ZP2和ZP3糖蛋白部分抑制精子与半透明带和卵母细胞的结合，并且HZA可作为内部对照的同源生物测定法，以评估透明带蛋白对精子与透明带紧密结合的影响。