巴西宫颈标本沙眼衣原体的基因分型
Genotyping of Chlamydia trachomatis from endocervical specimens in Brazil.
作者信息
Lima Haleta E, Oliveira Milena B, Valente Brenda G, Afonso Daniela A F, Darocha Wanderson D, Souza Maria Carmo M, Alvim Túlio C, Barbosa-Stancioli Edel F, Noronha Fátima Soares Motta
机构信息
Departamento de Microbiologia, Universidade Federal de Minas Gerais, Brasil.
出版信息
Sex Transm Dis. 2007 Sep;34(9):709-17. doi: 10.1097/01.olq.0000258399.27873.d9.
BACKGROUND
There is no data concerning genotyping of Chlamydia trachomatis from Brazilian samples.
GOAL
To characterize the genotype of C. trachomatis detected in women assisted at a STD public clinic and establish the prevalence of this infection in that population.
STUDY DESIGN
Endocervical samples of a group of 100 women were tested for chlamydial infection with PCR directed to C. trachomatis cryptic plasmid. Genotyping of positive samples were done after omp1 amplification and sequencing.
RESULTS
The overall prevalence of C. trachomatis infection was 19%, with the highest prevalence in women between 15 and 25 years old (68.4%). Four genotypes were found associated with endocervical infections: D, E, F, and K. Sequence analysis revealed a coinfection of genotypes D and E in 1 woman.
CONCLUSIONS
To our knowledge this is the first study to characterize Brazilian C. trachomatis endocervical samples and Brazilian C. trachomatis genotype coinfection. Our results also emphasize the importance of routine diagnosis of C. trachomatis for the control of this STD.
背景
目前尚无关于巴西样本中沙眼衣原体基因分型的数据。
目的
对在一家性传播疾病公共诊所接受治疗的女性中检测到的沙眼衣原体基因型进行特征分析，并确定该人群中这种感染的患病率。
研究设计
对一组100名女性的宫颈样本进行检测，采用针对沙眼衣原体隐蔽质粒的聚合酶链反应（PCR）检测衣原体感染。在进行omp1扩增和测序后，对阳性样本进行基因分型。
结果
沙眼衣原体感染的总体患病率为19%，其中15至25岁女性的患病率最高（68.4%）。发现有四种基因型与宫颈感染相关：D、E、F和K。序列分析显示1名女性存在基因型D和E的混合感染。
结论
据我们所知，这是第一项对巴西沙眼衣原体宫颈样本和巴西沙眼衣原体基因型混合感染进行特征分析的研究。我们的结果还强调了沙眼衣原体常规诊断对于控制这种性传播疾病的重要性。
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