Zheng Yue-zhong, Yuan Li-li, Lin Jin-yong, Li Hui-qiang
Tianjin Eye Hospital, Tianjin 300020, China.
Zhonghua Yan Ke Za Zhi. 2006 Dec;42(12):1118-22.
The aim was to investigate the suppression of rat experimental autoimmune uveoretinitis( EAU) by induced immune tolerance via intranasal administration of retinal S antigens.
The bovine S antigen was purified from bovine retina by salt precipitation and ionic exchange chromatography, the female Lewis rats were used to induce immune tolerance by intranasal administration with purified bovine retinal S antigens and then the rats were used to produce the EAU model by retinal S antigens challenge. The rate of EAU occurrence, the clinical and histological scores, the skin delayed-type hypersensitivity and lymphocyte proliferation stimulated by retinal S antigen and concanavalin A were recorded. The adjunct effect of cyclophosphamide on tolerance induction was observed.
After intranasal administration of retinal S antigens, EAU was induced in two of eight ( 25% ) rats in tolerant group, sis of six (100%) rats in control group , the difference of EAU induction rate was significant in tolerant group compared with control (P = 0. 0097) . The average onset time in tolerant group were 16. 5 days, the control group was 10. 3 days, the difference was significant ( F = 26. 32, P = 0. 000; q = 9. 723, P <0. 01). The average clinical scores of EAU in tolerant group were 0. 89, the control group was 3. 94, the difference was significant( F = 12. 48 ,P = 0. 000; q = 7. 904, P < 0. 01 ). The average histological scores of EAU in tolerant group were 1. 21, the control group was 4. 12, the difference was significant( F = 11. 80, P = 0. 000; q = 7. 510,P <0. 01). The histological features in tolerant group were iris blood vessels slightly dilation, few exudates in anterior chamber and vitreous cavity; there were slighter retina swallow and the photoreceptors damages in the tolerant group. The skin delayed-type hypersensitivity and the proliferative responses of lymphocytes stimulated by S antigen and concanavalin A in tolerant group were slighter than that in the control group. Intraperitoneal injection of cyclophosphamide enhanced the effect of immune tolerance slightly. Only intraperitoneal injection of cyclophosphamide did not diminish the severity of the rat EAU.
The intranasal induced tolerance by retinal S antigens can suppress effectively the prevalence of rat experimental autoimmune uveoretinitis induced by retinal S antigens.
通过鼻腔给予视网膜S抗原诱导免疫耐受，研究其对大鼠实验性自身免疫性葡萄膜视网膜炎（EAU）的抑制作用。
采用盐析和离子交换色谱法从牛视网膜中纯化牛S抗原，用纯化的牛视网膜S抗原经鼻腔给予雌性Lewis大鼠诱导免疫耐受，然后用视网膜S抗原攻击大鼠制备EAU模型。记录EAU发生率、临床和组织学评分、视网膜S抗原和刀豆蛋白A刺激的皮肤迟发型超敏反应及淋巴细胞增殖情况。观察环磷酰胺对耐受诱导的辅助作用。
鼻腔给予视网膜S抗原后，耐受组8只大鼠中有2只（25%）诱导出EAU，对照组6只大鼠中有6只（100%）诱导出EAU，耐受组与对照组EAU诱导率差异有统计学意义（P = 0. 0097）。耐受组平均发病时间为16. 5天，对照组为10. 3天，差异有统计学意义（F = 26. 32，P = 0. 000；q = 9. 723，P <0. 01）。耐受组EAU平均临床评分为0. 89，对照组为3. 94，差异有统计学意义（F = 12. 48，P = 0. 000；q = 7. 904，P <0. 01）。耐受组EAU平均组织学评分为1. 21，对照组为4. 12，差异有统计学意义（F = 11. 80，P = 0. 000；q = 7. 510，P <0. 01）。耐受组组织学特征为虹膜血管轻度扩张，前房和玻璃体腔少量渗出；视网膜水肿及光感受器损伤较轻。耐受组皮肤迟发型超敏反应及S抗原和刀豆蛋白A刺激的淋巴细胞增殖反应较对照组轻。腹腔注射环磷酰胺可轻微增强免疫耐受效果。单纯腹腔注射环磷酰胺不能减轻大鼠EAU的严重程度。
视网膜S抗原经鼻腔诱导的耐受可有效抑制视网膜S抗原诱导的大鼠实验性自身免疫性葡萄膜视网膜炎的发生。
