Thurau S R, Caspi R R, Chan C C, Weiner H L, Nussenblatt R B
Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892.
Fortschr Ophthalmol. 1991;88(4):404-7.
Experimental autoimmune uveitis (EAU) is induced in rats by active immunization with retinal S-Antigen (S-Ag) in complete Freund's adjuvant. In contrast, oral administration of an antigen induces specific suppression. We investigated the potential of oral administration of S-Ag for the treatment of EAU. Prior to active immunization rats received 3 x 1 mg S-Ag orally. In contrast to untreated controls, the treated rats were significantly protected from EAU, as determined by clinical and histological examination. Lymphocytes from treated rats showed no proliferative response to S-Ag in vitro, but had a normal proliferative response to other antigens. CD8-positive T cells from S-Ag-fed rats inhibited the proliferation of an antigen-specific uveitogenic T-cell line in vitro. Moreover, the intensity of the inflammatory reaction to active immunization could be suppressed when feeding was started after the active immunization. These data show, that oral administration induced suppression, which provided significant protection from the development of uveitis.
通过在完全弗氏佐剂中用视网膜S抗原（S-Ag）主动免疫诱导大鼠实验性自身免疫性葡萄膜炎（EAU）。相比之下，口服抗原可诱导特异性抑制。我们研究了口服S-Ag治疗EAU的潜力。在主动免疫前，大鼠口服3×1mg S-Ag。与未治疗的对照组相比，经临床和组织学检查确定，治疗组大鼠受到显著保护，未发生EAU。治疗组大鼠的淋巴细胞在体外对S-Ag无增殖反应，但对其他抗原有正常的增殖反应。来自喂食S-Ag大鼠的CD8阳性T细胞在体外抑制了抗原特异性致葡萄膜炎T细胞系的增殖。此外，在主动免疫后开始喂食时，对主动免疫的炎症反应强度可被抑制。这些数据表明，口服诱导的抑制作用可显著保护机体免受葡萄膜炎的发生。
