微阵列：质量控制与杂交方案。

Microarrays: quality control and hybridization protocol.

作者信息

Uno Ken-ichiro, Ueda Hiroki R

机构信息

Functional Genomics Subunit, Center for Developmental Biology, RIKEN, Kobe, Hyogo, Japan.

出版信息

Methods Mol Biol. 2007;362:225-43.

PMID:17417013

Abstract

Microarray technology is an exciting and promising tool, and is increasingly employed for studying circadian rhythms. To obtain optimal results from this technology, it is important to perform quality control experiments before engaging in genome-wide expression analysis. In this chapter, we provide an overview of quantitative polymerase chain reaction experiments using the ABI PRISM 7900HT system for quality control of samples, and microarray experiments using the Affymetrix GeneChip system for genome-wide expression analysis.

摘要

微阵列技术是一种令人兴奋且前景广阔的工具，并且越来越多地用于研究昼夜节律。为了从该技术中获得最佳结果，在进行全基因组表达分析之前进行质量控制实验非常重要。在本章中，我们概述了使用ABI PRISM 7900HT系统进行定量聚合酶链反应实验以对样品进行质量控制，以及使用Affymetrix GeneChip系统进行微阵列实验以进行全基因组表达分析。