Kramer Cas, Crosthwaite Susan K
Department of Genetics, University of Leicester, UK.
Methods Mol Biol. 2007;362:329-42. doi: 10.1007/978-1-59745-257-1_23.
In Northern analysis the presence of specific RNA transcripts is detected and their quantity can be estimated. RNA is separated using denaturing agarose gel electrophoresis and is subsequently transferred and fixed to a solid support, such as a nitrocellulose filter. When labeled probes are hybridized to these immobilized RNA molecules, their presence can be visualized by autoradiography. Here we describe Northern hybridization using radioactively labeled riboprobes to show circadian expression of endogenous sense and antisense frequency RNA in the filamentous fungus Neurospora crassa.
在Northern印迹分析中，可以检测到特定RNA转录本的存在，并可估计其数量。使用变性琼脂糖凝胶电泳分离RNA，随后将其转移并固定到固相支持物上，如硝酸纤维素滤膜。当标记的探针与这些固定的RNA分子杂交时，通过放射自显影可以观察到它们的存在。在这里，我们描述了使用放射性标记的核糖探针进行Northern杂交，以显示丝状真菌粗糙脉孢菌中内源性正义和反义频率RNA的昼夜节律表达。
