Llopis Xavier, Ibañez-García Nuria, Alegret Salvador, Alonso Julian
Grup de Sensors i Biosensors, Departament de Química Analítica, Edifici C, Universitat Autonoma de Barcelona, 08193 Cerdanyola del Vallés, Barcelona, Spain.
Anal Chem. 2007 May 15;79(10):3662-6. doi: 10.1021/ac062390z. Epub 2007 Apr 7.
Among the several fabrication techniques used to construct microflow systems, the low-temperature cofired ceramics (LTCC) technology, taking advantage of its multilayer approach, is one of the most versatile ones. It permits the integration of several unitary operations of an analytical process in a modular or monolithic way. Moreover, due to its perfect compatibility with screen-printing techniques, it also permits the integration of electronic components used to control the whole system setup. In this work the design, construction, and evaluation of a miniaturized analyzer for pesticide determination that integrates a pretreatment stage, based on two mixers or reactors, and an amperometric detection system to measure the product of an enzymatic inhibition reaction are presented. The detection system was monolithically integrated in the microfluidic platform, and it consisted of a screen-printed reference electrode and two platinum sheets, acting as auxiliary and working electrodes, which were embedded within the ceramic structure. The miniaturized system was characterized and successfully evaluated by determining carbofuran at the nanomolar level.
在用于构建微流控系统的几种制造技术中，低温共烧陶瓷（LTCC）技术凭借其多层方法，是用途最广泛的技术之一。它允许以模块化或整体式方式集成分析过程的多个单元操作。此外，由于其与丝网印刷技术的完美兼容性，它还允许集成用于控制系统设置的电子元件。在这项工作中，展示了一种用于农药测定的小型分析仪的设计、构建和评估，该分析仪集成了基于两个混合器或反应器的预处理阶段以及用于测量酶抑制反应产物的安培检测系统。检测系统整体集成在微流控平台中，它由一个丝网印刷参比电极和两个铂片组成，这两个铂片作为辅助电极和工作电极，嵌入在陶瓷结构内。通过在纳摩尔水平测定克百威对该小型化系统进行了表征和成功评估。
