Liang Yan, Yang Zai-Xing, Zhu Ye, Wang Yan, Zeng Xian-Ming, Deng An-Mei, Zhong Ren-Qian
Department of Laboratory Diagnostics and Clinical Immunology Center of PLA, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Zhonghua Yi Xue Za Zhi. 2007 Jan 9;87(2):128-30.
To explore the relationship between expression level of B cell-activating factor belonging to the TNF family (BAFF) receptor (BAFF-R) gene family and primary biliary cirrhosis (PBC).
With 18S rRNA as control, real-time fluorescent semi-quantification reverse transcriptional PCR was established, according to the difference of threshold cycles (DeltaCt) of target genes and 18S rRNA. B cell maturation antigen (BCMA), transmembrane activator and CAMI-interactor (TACI) and BAFF-R mRNA of peripheral blood mononuclear cells (PBMCs) in 30 healthy people and 30 PBC patients were measured. The correlation between their gene expression levels and the concentrations of IgM, ALP and GGT was analyzed.
The relative expression level of BCMA mRNA in patients with PBC was 8.6 folds higher than that of control (P < 0.05). In contrast, PBC patients showed relatively lower TACI mRNA level than control. For BAFF-R mRNA, there was no significant difference between patients and control. The gene expression of BCMA showed a close correlation with IgM, GGT and ALP (all P < 0.01), while BAFF-R showed no significant correlation with them (all P > 0.05). The correlation between TACI and IgM was significant (P < 0.05), but not for GGT or ALP (both P > 0.05).
The gene expression of PBMCs in PBC patients is significantly elevated for BCMA and decreased for TACI, indicating that the pathogenesis of PBC is closely related to enhanced humoral immunity and broken tolerance.
探讨肿瘤坏死因子家族B细胞活化因子（BAFF）受体（BAFF-R）基因家族表达水平与原发性胆汁性肝硬化（PBC）的关系。
以18S rRNA为对照，根据靶基因和18S rRNA的阈值循环差异（DeltaCt）建立实时荧光半定量逆转录PCR。检测30例健康人和30例PBC患者外周血单个核细胞（PBMC）中B细胞成熟抗原（BCMA）、跨膜激活剂和CAMI相互作用分子（TACI）以及BAFF-R mRNA水平。分析其基因表达水平与IgM、碱性磷酸酶（ALP）和γ-谷氨酰转肽酶（GGT）浓度的相关性。
PBC患者BCMA mRNA相对表达水平比对照组高8.6倍（P<0.05）。相反，PBC患者TACI mRNA水平相对低于对照组。对于BAFF-R mRNA，患者与对照组之间无显著差异。BCMA基因表达与IgM、GGT和ALP均密切相关（均P<0.01），而BAFF-R与它们均无显著相关性（均P>0.05）。TACI与IgM之间存在显著相关性（P<0.05），但与GGT或ALP无相关性（均P>0.05）。
PBC患者PBMC中BCMA基因表达显著升高，TACI基因表达降低，表示PBC的发病机制与体液免疫增强和耐受性破坏密切相关。
