Ito K, Hasegawa A, Komori S, Koyama K
Department of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan.
J Reprod Immunol. 2007 Aug;75(1):32-9. doi: 10.1016/j.jri.2007.03.001. Epub 2007 Apr 6.
Male reproductive tract CD52 (mrt-CD52) is known to be a pathogenic antigen for immunological infertility. Although human CD52 has been extensively investigated, the properties of mouse CD52 are not well elucidated. This study was conducted, therefore, to examine the tissue distribution, molecular composition and immunogenicity of mouse mrt-CD52. Immunohistological studies with an antibody to a synthetic peptide showed that mouse CD52 was localized mainly in the cauda epididymis and vas deferens, but not in the testis, liver, kidney or spleen. The molecule was composed of Asn (N)-linked and The/Ser (O)-linked carbohydrates as well as a glycosylphosphatidyl (GPI) anchor portion. Purified mrt-CD52 preparations produced antibodies by subcutaneous and intranasal immunization in both male and female mice. These antisera showed sperm-immobilizing activities with complement to mouse sperm. The research indicated mouse CD52 had similar biochemical and immunological properties to human CD52. This animal experiment is a good model for investigating human mrt-CD52 antibody detected in infertile patients.
雄性生殖道CD52（mrt-CD52）被认为是免疫性不育的致病抗原。尽管人类CD52已得到广泛研究，但小鼠CD52的特性尚未得到充分阐明。因此，本研究旨在检测小鼠mrt-CD52的组织分布、分子组成和免疫原性。用针对合成肽的抗体进行免疫组织学研究表明，小鼠CD52主要定位于附睾尾部和输精管，而在睾丸、肝脏、肾脏或脾脏中未检测到。该分子由天冬酰胺（N）连接和苏氨酸/丝氨酸（O）连接的碳水化合物以及糖基磷脂酰肌醇（GPI）锚定部分组成。纯化的mrt-CD52制剂通过皮下和鼻内免疫在雄性和雌性小鼠中均产生了抗体。这些抗血清对小鼠精子具有补体介导的精子固定活性。研究表明，小鼠CD52与人CD52具有相似的生化和免疫特性。该动物实验是研究在不育患者中检测到的人类mrt-CD52抗体的良好模型。
