Inagaki Yoshinori, Tang Wei, Guo Qian, Kokudo Norihiro, Sugawara Yasuhiko, Karako Hirona, Konishi Toshiro, Nakata Munehiro, Nagawa Hirokazu, Makuuchi Masatoshi
Hepato-Biliary-Pancreatic Surgery Division, Department of Surgery, Graduate School of Medicine, the University of Tokyo, Tokyo, Japan.
Hepatogastroenterology. 2007 Jan-Feb;54(73):53-7.
BACKGROUND/AIMS: Aberrant cell surface glycosylation, and especially excessive sialylation, is thought to have great importance in tumor malignancy. To investigate the clinicopathological significance of sialylation in colorectal cancer, we performed histochemical analyses using sialic acid-binding lectins.
Primary colorectal cancer and lymph node tissues obtained from 80 cases were subjected to lectin-immunohistochemical staining using Maackia amurensis leukoagglutinin (MAL) and Sambucus nigra agglutinin (SNA). The relationship between the staining characteristics and the various clinicopathological parameters was statistically analyzed.
In primary cancer tissues, a high level of SNA staining was significantly related to worse prognosis and some pathological characteristics such as lymph node metastasis, whereas a high level of MAL staining was related to worse prognosis. In metastatic lymph node tissues, positive staining was frequently found for MAL and SNA, which wasremarkable in cases categorized as N2 metastasis. Furthermore, cases with MAL-positive staining in metastatic lymph node tissues evidently indicated worse prognosis than those with MAL-negative staining.
Aberrant expression of SNA-positive sialoglycoconjugates in primary colorectal cancer tissues is important in terms of unfavorable pathological characteristics of the cancer. In addition, aberrant expression of MAL-positive sialoglycoconjugates in metastatic lymph node tissues might be related to worse prognosis.
背景/目的：异常的细胞表面糖基化，尤其是过度的唾液酸化，被认为在肿瘤恶性进展中具有重要意义。为了研究唾液酸化在结直肠癌中的临床病理意义，我们使用唾液酸结合凝集素进行了组织化学分析。
对80例患者的原发性结直肠癌和淋巴结组织进行了使用黑果腺肋花楸凝集素（MAL）和接骨木凝集素（SNA）的凝集素免疫组织化学染色。对染色特征与各种临床病理参数之间的关系进行了统计学分析。
在原发性癌组织中，高水平的SNA染色与较差的预后以及一些病理特征如淋巴结转移显著相关，而高水平的MAL染色与较差的预后相关。在转移性淋巴结组织中，经常发现MAL和SNA呈阳性染色，在归类为N2转移的病例中尤为明显。此外，转移性淋巴结组织中MAL阳性染色的病例的预后明显比MAL阴性染色的病例差。
原发性结直肠癌组织中SNA阳性唾液酸糖缀合物的异常表达对于癌症的不良病理特征具有重要意义。此外，转移性淋巴结组织中MAL阳性唾液酸糖缀合物的异常表达可能与较差的预后相关。
