Klaniecki J, Dykers T, Travis B, Schmitt R, Wain M, Watson A, Sridhar P, McClure J, Morein B, Ulrich J T
Bristol-Myers Squibb Pharmaceutical Research Institute Seattle, WA.
AIDS Res Hum Retroviruses. 1991 Oct;7(10):791-8. doi: 10.1089/aid.1991.7.791.
A recombinant vaccinia virus in which the transcription of the human immunodeficiency virus type 1 (BRU isolate) env gene is driven by the 11K late vaccinia promoter yields about 10-fold higher amounts of gp160 env protein upon infection of monkey cells than does a recombinant in which gp160 is expressed using the 7.5K early-late promoter. The gp160 was purified from detergent lysates of infected cells by lentil lectin affinity chromatography followed by immunoaffinity chromatography, and was obtained in yields of 1-2 mg/10(9) cells of material estimated to be about 70% pure. Pairs of rabbits were immunized with purified gp160 using either one of five different adjuvants or an immunostimulating complex. In all cases a substantial humoral immune response was obtained after boosting, including an activity that neutralized the homologous (BRU) isolate of HIV-1. In some cases, this activity also neutralized two distantly related isolates, SF2 and MN.
一种重组痘苗病毒，其中1型人类免疫缺陷病毒（BRU分离株）env基因的转录由11K晚期痘苗启动子驱动，在感染猴细胞时产生的gp160 env蛋白量比使用7.5K早期-晚期启动子表达gp160的重组病毒高出约10倍。通过扁豆凝集素亲和层析，然后免疫亲和层析，从感染细胞的去污剂裂解物中纯化gp160，以1-2 mg/10⁹个细胞的产量获得估计纯度约为70%的材料。用五种不同佐剂之一或免疫刺激复合物对兔进行纯化gp160免疫。在所有情况下，加强免疫后均获得了显著的体液免疫反应，包括中和HIV-1同源（BRU）分离株的活性。在某些情况下，这种活性还中和了两个远缘相关分离株SF2和MN。
