血清孕酮测定辅助非繁殖季节配种山羊的妊娠诊断。
Serum progesterone determination as an aid for pregnancy diagnosis in goats bred out of season.
作者信息
Fleming S A, Van Camp S D, Chapin H M
出版信息
Can Vet J. 1990 Feb;31(2):104-7.
Abstract
Nubian does (n = 12) were bred by artificial insemination after induction of estrus with medroxyprogesterone acetate impregnated vaginal sponges and pregnant mare serum gonadotrophin injections during the anestrous season. Pregnancy status was predicted from serum samples collected 21 days following the last breeding and analyzed using 1) a commercial bovine milk progesterone enzyme immunoassay test (EIA), and 2) a radioimmunoassay progesterone (RIA) test. Both tests detected nonpregnancy (EIA 100%, RIA 80%) more accurately than pregnancy (EIA 66%, RIA 75%). Commercial bovine progesterone EIA kits have potential as rapid, inexpensive screening tests for nonpregnant does bred out of season.
摘要
在发情季节，给12只努比亚母羊植入醋酸甲羟孕酮浸渍的阴道海绵并注射孕马血清促性腺激素以诱导发情，然后进行人工授精。在最后一次配种后21天采集血清样本，通过以下两种方法分析来预测妊娠状态：1）使用商用牛乳孕酮酶免疫分析测试（EIA）；2）放射免疫分析孕酮（RIA）测试。两种测试检测未怀孕（EIA为100%，RIA为80%）比检测怀孕（EIA为66%，RIA为75%）更准确。商用牛孕酮EIA试剂盒有潜力作为快速、廉价的非季节性配种未怀孕母羊的筛查测试。
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Characterization of the caprine estrous cycle using enzyme immunoassay for the determination of whole blood progesterone concentration.利用酶免疫测定法测定全血孕酮浓度对山羊发情周期进行特征分析。
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