Yang Jaewook, Lee Dokyung, Kim Yuna, Kang Byungyong, Kim Kyungjae, Ha Namjoo
School of Pharmacy, Western University, Pomona, California, USA.
Arch Pharm Res. 2007 Mar;30(3):329-36. doi: 10.1007/BF02977614.
Enterococcus faecalis and Enterococcus faecium isolated from Korea patients in 1998 and 2005 were tested for susceptibility to nine different antimicrobial agents, including vancomycin and teicoplanin. Polymerase chain reaction (PCR) was used for the detection of several vancomycin resistance genes such as vanA ('high level'), vanB ('moderate high level'), vanC1 and vanC2 ('low level'). Both E. faecalis and E. faecium exhibited a resistance of 80% and more than 60% to synercid and mupirocin, respectively. Moreover, an average of 76% of all isolates was resistant to ciprofloxacin, gentamicin, lincomycin, cefotaxime, and meropenem, confirming the multiple drug resistance of most of the isolates. No resistance to vancomycin or teicoplanin was observed in the 1998 E. faecalis and E. faecium isolates. However, the 2005 E. faecalis and E. faecium isolates exhibited resistance of 16% and 12% to vancomycin and teicoplanin, respectively. In addition, vanA gene was detected in 4 strains of 2005 E. faecium isolates, thus showing a high resistance to vancomycin. No other vancomycin resistance genes, including vanB, vanC1, and vanC2, were found in our isolates. In this study, we compared the occurrence of antimicrobial resistance and the presence of specific resistance genes in E. faecalis and E. faecium. The results showed that the 2005 isolates were usually more resistant than the 1998 isolates.
对1998年和2005年从韩国患者中分离出的粪肠球菌和屎肠球菌进行了9种不同抗菌药物（包括万古霉素和替考拉宁）的敏感性测试。采用聚合酶链反应（PCR）检测几种万古霉素耐药基因，如vanA（“高水平”）、vanB（“中等高水平”）、vanC1和vanC2（“低水平”）。粪肠球菌和屎肠球菌对synercid和莫匹罗星的耐药率分别为80%和60%以上。此外，所有分离株平均76%对环丙沙星、庆大霉素、林可霉素、头孢噻肟和美罗培南耐药，证实大多数分离株具有多重耐药性。在1998年的粪肠球菌和屎肠球菌分离株中未观察到对万古霉素或替考拉宁的耐药性。然而，2005年的粪肠球菌和屎肠球菌分离株对万古霉素和替考拉宁的耐药率分别为16%和12%。此外，在2005年的4株屎肠球菌分离株中检测到vanA基因，因此对万古霉素表现出高耐药性。在我们的分离株中未发现其他万古霉素耐药基因，包括vanB、vanC1和vanC2。在本研究中，我们比较了粪肠球菌和屎肠球菌中抗菌药物耐药性的发生情况和特定耐药基因的存在情况。结果表明，2005年的分离株通常比1998年的分离株耐药性更强。
