Webb R, Mendez D, Berger L, Speare R
Amphibian Disease Ecology Group, School of Public Health, Tropical Medicine and Rehabilitation Sciences, James Cook University, Townsville 4811, Australia.
Dis Aquat Organ. 2007 Feb 8;74(1):13-6. doi: 10.3354/dao074013.
Chytridiomycosis, a disease contributing to amphibian declines worldwide, is caused by the fungus Batrachochytrium dendrobatidis. Identifying efficient and practical disinfectants effective against B. dendrobatidis is important to reduce the spread of the disease both in the wild and captivity. Previous studies identified a range of suitable disinfectant strategies. We evaluated the suitability of 3 additional disinfectants: two of these (TriGene Virucidal Disinfectant Cleaner and F10 Super Concentrate Disinfectant) are mixtures of chemicals and one (Betadine Antiseptic Liquid) contains a single active ingredient, povidone iodine. The disinfectants were tested using a range of concentrations for 1,5 and 10 min to determine their ability to kill B. dendrobatidis in vitro. The measure of effectiveness was 100% kill of zoosporangia grown in multiwell plates. All disinfectants had a 100% efficacy at concentrations recommended by the manufacturers. The lowest concentrations capable of 100% kill after exposure for 1 min were 0.1 ml l(-1) for TriGene, 0.33 ml l(-1) for F10 and 100 ml l(-1) for Betadine. TriGene is the most effective disinfectant yet to be found, and both TriGene and F10 are more effective than various disinfectants tested in previous studies. TriGene and F10 are considered suitable for use in the field, as only small amounts of concentrate are needed.
壶菌病是一种导致全球两栖动物数量减少的疾病，由真菌蛙壶菌引起。确定对蛙壶菌有效的高效实用消毒剂对于减少该疾病在野外和圈养环境中的传播至关重要。先前的研究确定了一系列合适的消毒策略。我们评估了另外三种消毒剂的适用性：其中两种（TriGene杀病毒消毒剂清洁剂和F10超级浓缩消毒剂）是化学混合物，另一种（碘伏消毒液）含有单一活性成分聚维酮碘。使用一系列浓度对消毒剂进行1、5和10分钟的测试，以确定它们在体外杀死蛙壶菌的能力。有效性的衡量标准是在多孔板中生长的游动孢子囊100%被杀死。所有消毒剂在制造商推荐的浓度下均具有100%的功效。暴露1分钟后能够100%杀死病菌的最低浓度，TriGene为0.1 ml l(-1)，F10为0.33 ml l(-1)，碘伏为100 ml l(-1)。TriGene是迄今发现的最有效的消毒剂，并且TriGene和F10都比先前研究中测试的各种消毒剂更有效。TriGene和F10被认为适用于野外使用，因为只需要少量浓缩液。
