Feng Jian-yong, Zhu Quan-hong, Luo Jia-bo
School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2007 Mar;27(3):268-71.
To investigate the optimal condition for complete removal of the template molecules from vinblastine (VLB)-imprinted polymer.
The prepared polymers were packed into the cartridges of solid-phase extraction column and washed by methanol-glacial acetic acid mixture with different proportions. The contents and recoveries of VLB in the effluents were determined.
Polymer extraction with methanol-glacial acetic acid (9:1, V/V) resulted in VLB recovery of 91.73%, but template bleeding was observed because of incomplete VLB removal. Using methanol-glacial acetic acid (6:4, V/V) as the extraction solvent, the recovery of VLB reached 98.03% with less solvents and extract times. The polymers could selectively adsorb VLB through non-covalent interactions and still exhibited strong affinity for the template molecule but not for the structural analogue vincristine after extraction with methanol-glacial acetic acid (6:4, V/V).
Methanol-glacial acetic acid (6:4, V/V) is an ideal extract solvent for complete template molecule removal from the polymers, and the processed polymers possess stable capacity of specific recognition and selectivity to the template.
研究从长春碱（VLB）印迹聚合物中完全去除模板分子的最佳条件。
将制备的聚合物填充到固相萃取柱的小柱中，并用不同比例的甲醇 - 冰醋酸混合物洗涤。测定流出物中VLB的含量和回收率。
用甲醇 - 冰醋酸（9:1，V/V）萃取聚合物时，VLB回收率为91.73%，但由于VLB去除不完全，观察到模板渗漏。以甲醇 - 冰醋酸（6:4，V/V）作为萃取溶剂，VLB回收率达到98.03%，且溶剂用量和萃取次数更少。用甲醇 - 冰醋酸（6:4，V/V）萃取后，聚合物可通过非共价相互作用选择性吸附VLB，对模板分子仍表现出强亲和力，但对结构类似物长春新碱则无亲和力。
甲醇 - 冰醋酸（6:4，V/V）是从聚合物中完全去除模板分子的理想萃取溶剂，处理后的聚合物对模板具有稳定的特异性识别和选择性能力。
