Zhang Wei-sen, Xu Qi-rong, Jiang Chao-qiang, Chen Qing
Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2007 Mar;27(3):286-9.
To establish a method of ion chromatography for urine iodide determination and evaluate its application in epidemiological studies.
Urinary iodine was determined using ICS-2500 ion chromatography coupled with IonPac AS7 analytical column (4 mm x 250 mm), silver electrode and direct current amperometry for 467 elderly subjects (above 50 years) with their urinary Tsh and FT4 also determined. The history of thyroid diseases was examined among these subjects.
The peak area of the ion chromatography was linearly correlated with urinary iodine concentration, with the correlation coefficient of 0.9999. The detection limit of iodide was around 3.5 microg/L (S/N=3), with coefficients of variation ranging from 3.16% to 3.45% and mean recovery rate of 95%. The urinary iodine level in the 467 elderly subjects showed a positively skewed distribution with the median level of 224.7 microg/L.
Ion chromatography for urinary iodine determination has excellent selectivity, sensitivity, reliability, accuracy and stability, and may ensure satisfactory effect for application in epidemiological studies.
建立离子色谱法测定尿碘的方法并评估其在流行病学研究中的应用。
采用ICS - 2500离子色谱仪，结合IonPac AS7分析柱（4 mm×250 mm）、银电极和直流安培法，对467名50岁以上老年受试者的尿碘进行测定，并同时测定其尿促甲状腺激素（Tsh）和游离甲状腺素（FT4）。对这些受试者进行甲状腺疾病史调查。
离子色谱峰面积与尿碘浓度呈线性相关，相关系数为0.9999。碘化物的检测限约为3.5μg/L（信噪比=3），变异系数在3.16%至3.45%之间，平均回收率为95%。467名老年受试者的尿碘水平呈正偏态分布，中位数水平为224.7μg/L。
离子色谱法测定尿碘具有良好的选择性、灵敏度、可靠性、准确性和稳定性，可确保在流行病学研究中的应用效果令人满意。
