Cation Cl- cotransporters in the dendrites of goldfish bipolar cells.

Bipolar cells receive an input from gamma-aminobutyric acid ergic horizontal cells in distal retinas. gamma-Aminobutyric acid can induce either an inhibitory or an excitatory Cl(-) response based on the intracellular Cl(-) concentration in the bipolar dendrites. Two cation Cl(-) cotransporters, Na-K-2Cl(-) and K-2Cl(-), differently control local Cl(-) levels in neurons by uptaking or extracting Cl(-), respectively. By using immunocytochemical techniques, we found that Na-K-2Cl(-) and K-2Cl(-) were preferentially distributed in On-bipolar and Off-bipolar dendrites in goldfish retinas. Na-K-2Cl(-) was colocalized with protein kinase C in On-bipolar dendrites, whereas K-2Cl(-) was located in Off-bipolar dendrites, fully overlapping with ionotropic glutamate receptor GluR4. Possibly, an internal Cl(-) level is higher in the On-bipolar dendrites than in the Off-bipolar dendrites. gamma-Aminobutyric acid inputs in the distal retina might excite On-bipolar cells, but inhibit Off-bipolar cells.