Munaut C, Noël A, Sobel M, Foidart J M
Laboratory of Biology, University of Liege, Belgium.
Cell Biol Int Rep. 1991 Jun;15(6):499-509. doi: 10.1016/0309-1651(91)90119-4.
We have cultured normal fibroblasts, fibrosarcoma HT1080 cells and breast adenocarcinoma MCF-7 cells on various substrates (plastic, collagen type I, laminin). All cell types used adhered on the three substrates with, however, a delayed attachment on laminin. On all substrates, cell grew as monolayer with the exception of MCF-7 cells that formed clusters on laminin. The epithelial MCF-7 cells as well as mesenchymal cells (fibroblasts and tumoral HT1080 cells) synthesized laminin and expressed mRNA coding for laminin B1 chain and for the 67 kD laminin binding protein. The levels of these mRNAs were not modulated by culture conditions which affect cell morphology nor by cell density.
我们已将正常成纤维细胞、纤维肉瘤HT1080细胞和乳腺腺癌MCF-7细胞培养于各种基质（塑料、I型胶原、层粘连蛋白）上。所用的所有细胞类型均能附着于这三种基质上，不过，在层粘连蛋白上的附着有所延迟。在所有基质上，细胞均呈单层生长，但MCF-7细胞在层粘连蛋白上形成细胞簇除外。上皮性MCF-7细胞以及间充质细胞（成纤维细胞和肿瘤性HT1080细胞）合成层粘连蛋白，并表达编码层粘连蛋白B1链和67kD层粘连蛋白结合蛋白的mRNA。这些mRNA的水平不受影响细胞形态的培养条件调控，也不受细胞密度的调控。
