Marsh Victoria, Clarke Alan
Cardiff University, Cardiff School of Biosciences, Cardiff, UK.
Expert Rev Anticancer Ther. 2007 Apr;7(4):519-31. doi: 10.1586/14737140.7.4.519.
Constitutive mouse models of intestinal neoplasia, such as the Apc(min/+) (multiple intestinal neoplasia) mouse have proven valuable tools both for furthering our understanding of tumorigenesis and for the development of therapeutic strategies. However, the in vivo study of a number of genes has been precluded by their absolute requirement during embryonic development. This has led to the development of conditional strategies that allow gene regulation in vivo. This review describes the principal techniques used to achieve conditional transgenesis within the mouse intestine, with a particular focus upon the Cre-Lox and Tet-regulable systems. Further, we discuss how these techniques are being used to dissect the mechanisms governing both normal homeostasis and neoplastic development within the intestine.
肠道肿瘤形成的组成型小鼠模型，如Apc（min/+）（多发性肠道肿瘤）小鼠，已被证明是推进我们对肿瘤发生的理解以及开发治疗策略的宝贵工具。然而，由于许多基因在胚胎发育过程中的绝对需求，对它们的体内研究受到了限制。这导致了允许体内基因调控的条件策略的发展。本综述描述了在小鼠肠道内实现条件转基因的主要技术，特别关注Cre-Lox和Tet可调控系统。此外，我们讨论了这些技术如何被用于剖析肠道内正常稳态和肿瘤发生的调控机制。
