Lin Yingbo, Zhan Wenbin, Li Qiang, Zhang Zhidong, Wei Xiumei, Sheng Xiuzhen
Laboratory of Pathology and Immunology of Aquatic Animals, LMMEC, Ocean University of China, No. 5, Yushan Road, Qingdao 266003, PR China.
Dev Comp Immunol. 2007;31(11):1073-81. doi: 10.1016/j.dci.2007.02.001. Epub 2007 Mar 6.
Monoclonal antibodies (Mabs) specifically against haemocytes of shrimp (Fenneropenaeus chinensis) were employed to study the ontogenesis of haemocytes by immunofluorescence assay technique (IFAT) with the aids of avidin-biotin complex method. Different developmental stages were identified by means of histochemical staining. Stages including blastula, gastrula, limb bud stage, embryonic-nauplius and nauplius were examined. With Mab 2A3 against both hyline, semi-granular and granular haemocytes, the first positive cells were detected at the limb bud stage of the embryos. Distinguishable positive haemocytes with a diameter of about 7 microm appeared in embryonic-nauplius. Whereas, with Mab 1H11 specific to cytoplasmic granulars in haemocytes, the first positive granular haemocytes were observed at the stage of embryonic-nauplius. Increased number of both total and granular haemocytes at the stage of nauplius impressed a possible enhancement of the immune system as the embryogenesis progressed.
利用特异性针对中国对虾血细胞的单克隆抗体，借助抗生物素蛋白-生物素复合物法，通过免疫荧光检测技术（IFAT）研究血细胞的个体发生。通过组织化学染色确定不同发育阶段。检查了囊胚、原肠胚、肢芽期、胚胎无节幼体和无节幼体等阶段。使用针对透明、半颗粒和颗粒血细胞的单克隆抗体2A3，在胚胎肢芽期检测到第一批阳性细胞。在胚胎无节幼体中出现了直径约7微米的可区分阳性血细胞。而对于特异性针对血细胞中细胞质颗粒的单克隆抗体1H11，在胚胎无节幼体阶段观察到第一批阳性颗粒血细胞。在无节幼体阶段，总血细胞和颗粒血细胞数量的增加表明随着胚胎发育的进行，免疫系统可能得到增强。
