Bretaud S, Li Q, Lockwood B L, Kobayashi K, Lin E, Guo S
Department of Biopharmaceutical Sciences, University of California, San Francisco, CA 94143-2811, USA.
Neuroscience. 2007 May 25;146(3):1109-16. doi: 10.1016/j.neuroscience.2006.12.073. Epub 2007 Apr 11.
Transparent larval zebrafish offer the opportunity to unravel genetic and neuronal networks underlying behavior in a developing system. In this study, we developed a choice chamber paradigm to measure reward-associated behavior in larval zebrafish. In the chamber where larval zebrafish have a choice of spending their time in either a water- or morphine-containing compartment, larvae that have previously experienced morphine spend significantly more time in the compartment containing morphine. This behavior can be attentuated by pre-treatment with antagonists of the opioid receptor or the dopamine receptor, and furthermore, is impaired in the too few mutant, which has a genetic deficiency in the production of specific groups of dopaminergic and serotonergic neurons in the ventral forebrain. These results uncover a choice behavior for an addictive substance in larval zebrafish that is mediated through central opioid and monoaminergic neurotransmitter systems.
透明的斑马鱼幼体为在发育系统中揭示行为背后的遗传和神经网络提供了机会。在本研究中，我们开发了一种选择腔室范式来测量斑马鱼幼体中与奖赏相关的行为。在斑马鱼幼体可以选择在含纯水或含吗啡的隔室中度过时间的腔室中，先前接触过吗啡的幼体在含吗啡的隔室中花费的时间明显更多。这种行为可通过用阿片受体或多巴胺受体拮抗剂进行预处理而减弱，此外，在too few突变体中受损，该突变体在前脑腹侧特定组的多巴胺能和5-羟色胺能神经元的产生方面存在基因缺陷。这些结果揭示了斑马鱼幼体中一种成瘾物质的选择行为，该行为是通过中枢阿片和单胺能神经递质系统介导的。
