[含HIV-1 gag的35型纤维嵌合腺病毒5型载体在小鼠中的免疫原性]
[Immunogenicity of a chimeric adenovirus type 5 vector with type 35 fiber containing HIV-1 gag in mice].
作者信息
Liu Xin-lei, Yu Shuang-qing, Feng Xia, Wang Xiao-li, Liu Hong-mei, Zhang Xiao-mei, Li Hong-xia, Zhou Ling, Li Ze-lin, Zheng Yi
机构信息
College of Life Science and Bio-engineering, Beijing University of Technology, Beijing 100022, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2007 Mar;21(1):5-7.
OBJECTIVE
To study the immune effect of a chimeric adenovirus type 5 vector with type 35 fiber (rAd5/F35) vaccine in BALB/c mice.
METHODS
The expression of HIV Gag protein was determined using indirect immunofluorescent staining. The rAd5/F35-mod.gag vector was injected intramuscularly to mice. The IgG antibody was detected by ELISA and CTL response was detected by intracellular cytokine stain assay.
RESULTS
The rAd5/F35-mod.gag vector could express HIV Gag protein in vitro and generate strong HIV-specific immune responses in vivo. But anti-Ad5 immunity could limit its immunogenicity in vivo.
CONCLUSION
The rAd5/F35-mod.gag vector can elicit specific CTL response and IgG antibody in animal model. In mice with high Ad5 vector-specific immunity, Ad5/F35-mod.gag showed lower level of Gag specific CTL and antibody response than in mice without pre-existing adenovirus type 5 immunity. The results indicated that fiber exchange alone does not evade pre-existing Ad5 immunity.
目的
研究携带35型纤维的嵌合5型腺病毒载体（rAd5/F35）疫苗在BALB/c小鼠中的免疫效果。
方法
采用间接免疫荧光染色法测定HIV Gag蛋白的表达。将rAd5/F35-mod.gag载体肌肉注射到小鼠体内。通过ELISA检测IgG抗体，通过细胞内细胞因子染色试验检测CTL反应。
结果
rAd5/F35-mod.gag载体在体外可表达HIV Gag蛋白，并在体内产生强烈的HIV特异性免疫反应。但抗Ad5免疫可限制其在体内的免疫原性。
结论
rAd5/F35-mod.gag载体可在动物模型中引发特异性CTL反应和IgG抗体。在具有高Ad5载体特异性免疫的小鼠中，Ad5/F35-mod.gag的Gag特异性CTL和抗体反应水平低于无预先存在的5型腺病毒免疫的小鼠。结果表明，仅纤维交换不能逃避预先存在的Ad5免疫。
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