[一种通过在硅胶UV-254板上进行色谱分析测定腺苷酸环化酶活性的微量方法]
[A micromethod for determination of the adenylate cyclase activity by chromatography on silufol UV-254 plates].
作者信息
Beritashvili D R, Kafiana K A
出版信息
Vopr Med Khim. 1975 May-Jun;21(3):322-4.
PMID:174304
Abstract
A method is developed for micro-scale separation of the products of enzymatic degradation of ATP (ADP, 5'-AMP, adenosine 3',5'-cytophosphate, adenosine and adenine) by one-dimentional thin-layer chromatography on Silufol UV-254 plates. The method is applicable for determination of activity of adenylate cyclase and of other enzymes involved in metabolism of adenylic derivatives, including the diesterase of 3',5'-cyclic AMP.
摘要
开发了一种方法，用于在Silufol UV-254板上通过一维薄层色谱法对ATP酶促降解产物（ADP、5'-AMP、3',5'-环磷酸腺苷、腺苷和腺嘌呤）进行微尺度分离。该方法适用于测定腺苷酸环化酶以及参与腺苷酸衍生物代谢的其他酶的活性，包括3',5'-环磷酸腺苷二酯酶。
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