Kawamoto E, Okiyama E, Sasaki H, Sawada T, Mikazuki K, Ueshiba H
Animal Research Center, Tokyo Medical University, Shinjuku, Tokyo 160-8402, Japan.
Lab Anim. 2007 Apr;41(2):285-91. doi: 10.1258/002367707780378087.
The surface structures of the cells of Pasteurella pneumotropica from mice and Pasteurella multocida from rabbits were examined by transmission electron microscopy after ruthenium red staining and polycationic ferritin labelling. P. pneumotropica strains ATCC 35149 and K 79114 had slight extracellular fibrous materials associated with cell walls with ruthenium red staining. Ferritin labelling method revealed thick strands or sparsely ferritin-labelled materials on the cell surface of the strains. P. multocida strains Pm-78 and P-2440 had ferritin-labelled capsules surrounded with the cell wall. Strain Pm-78, which was serotyped as A:12, had a thick capsule, whereas serotype -:3 strain P-2440 had a thin and irregular capsule.
采用钌红染色和聚阳离子铁蛋白标记后，通过透射电子显微镜检查了来自小鼠的嗜肺巴斯德氏菌和来自兔子的多杀巴斯德氏菌细胞的表面结构。嗜肺巴斯德氏菌菌株ATCC 35149和K 79114经钌红染色后，细胞壁周围有轻微的细胞外纤维物质。铁蛋白标记法显示，这些菌株的细胞表面有粗链或铁蛋白标记稀疏的物质。多杀巴斯德氏菌菌株Pm - 78和P - 2440有被细胞壁包围的铁蛋白标记的荚膜。血清型为A:12的菌株Pm - 78有厚荚膜，而血清型-:3的菌株P - 2440有薄且不规则的荚膜。
