Rosen P, Boulton M, Moriarty P, Khaliq A, McLeod D
Moorfields Eye Hospital, London, U.K.
Exp Eye Res. 1991 Nov;53(5):597-601. doi: 10.1016/0014-4835(91)90218-4.
Microvascular fragments and pure cultures of either retinal microvascular endothelial cells, retinal pericytes or Tenon's capsule fibroblasts were grown on a gas permeable substrate while exposed to varying oxygen concentrations (5, 10, 20, 40 and 95% oxygen). For all three cell types cell proliferation was greatest under the lowest oxygen concentration and decreased as the oxygen concentration increased. The decrease in the proliferative ability of cells exposed to the higher oxygen concentrations could be reversed if the cells were returned to a normoxic environment. Endothelial cells were the most sensitive to changes in oxygen tensions showing a proliferative response after 24 hr exposure as opposed to 48 hr for pericytes and fibroblasts. These results suggest a direct mechanism by which the intra-vitreal and intra-retinal oxygen tension may influence both the development of preretinal new vessels (in response to retinal ischaemia) and also the regression of neovascularization following scatter photocoagulation or vitrectomy.
微血管片段以及视网膜微血管内皮细胞、视网膜周细胞或Tenon囊成纤维细胞的纯培养物，在透气基质上生长，同时暴露于不同的氧浓度（5%、10%、20%、40%和95%氧气）下。对于所有三种细胞类型，在最低氧浓度下细胞增殖最为显著，且随着氧浓度升高而降低。如果将暴露于较高氧浓度的细胞放回常氧环境，其增殖能力的下降可得到逆转。内皮细胞对氧张力变化最为敏感，暴露24小时后即出现增殖反应，而周细胞和成纤维细胞则为48小时。这些结果提示了一种直接机制，通过该机制玻璃体内和视网膜内的氧张力可能会影响视网膜前新血管的形成（对视网膜缺血的反应）以及散射光凝或玻璃体切除术后新生血管的消退。
