Stepanova I S, Bogoliubov D S, Parfenov V N
Tsitologiia. 2007;49(1):5-20.
Cajal bodies (CBs) in oocytes of the house cricket Acheta domesticus are large, perfectly spherical nuclear organelles with a complex internal structure. These consist of a fibrillar coilin-containing matrix and a central cavity with a prominent fibrogranular body inside; the latter has been referred to as an "internal" interchromatin granule cluster (IGC). Within the matrix of CBs we detected transcriptional co-activators CBP/p300 and TATA-box binding protein (TBP). No RNA polymerase II was revealed in CBs of both normal and actynomycin D treated oocytes. In the nucleoplasm of A. domesticus oocytes, besides CBs, free IGCs were observed. In oocytes treated with actynomycin D, the amount of "free" IGCs in the nucleoplasm increase significantly, granular and fibrillar components of IGCs were seen segregated, and RNA polymerase II and CBP/p300 were found to be accumulated in fibrillar zones of IGCs.
家蟋蟀（Acheta domesticus）卵母细胞中的卡哈尔体（CBs）是大型、完美球形的核细胞器，具有复杂的内部结构。这些结构由含卷曲螺旋蛋白的纤维状基质和一个中央腔组成，中央腔内有一个突出的纤维颗粒体；后者被称为“内部”染色质间颗粒簇（IGC）。在CBs的基质中，我们检测到转录共激活因子CBP/p300和TATA框结合蛋白（TBP）。在正常和放线菌素D处理的卵母细胞的CBs中均未发现RNA聚合酶II。在家蟋蟀卵母细胞的核质中，除了CBs外，还观察到游离的IGC。在用放线菌素D处理的卵母细胞中，核质中“游离”IGC的数量显著增加，IGC的颗粒和纤维成分被分离，并且发现RNA聚合酶II和CBP/p300积累在IGC的纤维区域。
