Shee Fabrice Lin Teng, Angers Paul, Bazinet Laurent
Institute of Nutraceuticals and Functional Foods and Dairy Research Group STELA, Department of Food Sciences and Nutrition, Laval University, Quebec G1K 7P4, Canada.
J Agric Food Chem. 2007 May 16;55(10):3985-9. doi: 10.1021/jf070191q. Epub 2007 Apr 14.
The separation of residual fats from whey protein concentrates (WPC) results in a better nutritional and functional utilization of this product. Bipolar membrane electroacidification (BMEA) technology allows acidification and demineralization of solutions without any salt addition. The principle of BMEA is based on proton formation from water molecule dissociation at the bipolar membrane interface. The objective of this work was to determine the effect of an electroacidification treatment at pH 4.5 on the precipitation of lipids. WPC electroacidification was carried out with or without preliminary demineralization by conventional electrodialysis. The effect of ionic strength on lipid precipitation rates was also evaluated by dilution of the WPC samples. Lipid precipitation levels of 35-39% were obtained using the electroacidification process without a dilution step, while the combination of BMEA and dilution of the WPC resulted in a decrease in lipid content by six-fold from 0.76 to 0.21%.
从乳清蛋白浓缩物(WPC)中分离残留脂肪可使该产品在营养和功能利用方面表现更佳。双极膜电酸化(BMEA)技术可在不添加任何盐分的情况下对溶液进行酸化和脱盐。BMEA的原理基于双极膜界面处水分子解离产生质子。本研究的目的是确定pH 4.5的电酸化处理对脂质沉淀的影响。WPC电酸化处理在有或没有通过传统电渗析进行初步脱盐的情况下进行。还通过稀释WPC样品评估了离子强度对脂质沉淀速率的影响。在不进行稀释步骤的情况下,采用电酸化工艺可使脂质沉淀水平达到35 - 39%,而BMEA与WPC稀释相结合则使脂质含量从0.76%降至0.21%,降低了六倍。