Hodaifa Gassan, Martínez M A Eugenia, Sánchez S
Complutense University of Madrid, Faculty of Chemical Science, Chemical Engineering, Ciudad Universitaria, s/n, 28040 Madrid, Spain.
Bioresour Technol. 2008 Mar;99(5):1111-7. doi: 10.1016/j.biortech.2007.02.020. Epub 2007 Apr 16.
The use of rinse water (RW) from two-phase centrifugation in the olive-oil extraction industry has been studied in relation to the production of the microalga Scenedesmus obliquus (CCAP 276/3A). The characterization of the wastewater indicates that it is N deficient. The highest value of maximum specific growth rate, 0.044 h(-1) was registered in the culture with 5% RW. However, biomass productivity proved greater in the culture with 100% RW. The specific growth rates were adjusted to a model of inhibition by substrate. The content in normalized chlorophyll diminished with the duration of the cultures, reaching a minimum CHL'(min) value, which showed an inhibition type similar to that of the specific growth rate. The yield in protein indicates that the cultures were limited by N to approximately 50% RW. The greater specific rate of protein synthesis during the exponential phase was 3.7 mg/gh to 50% RW. It was found that the composition of the lipid fraction of the biomass depended on the percentage of wastewater used as the nutrient medium, reaching the highest percentages of monounsaturated, polyunsaturated, and essential fatty acids in the culture with 100% RW.
针对斜生栅藻（CCAP 276/3A）的培养，研究了橄榄油提取行业中两相离心法产生的冲洗水（RW）的利用情况。废水特性表明其氮含量不足。在含5% RW的培养物中，最大比生长速率达到最高值0.044 h⁻¹。然而，含100% RW的培养物中生物量生产力更高。比生长速率符合底物抑制模型。归一化叶绿素含量随培养时间减少，达到最低CHL'(min)值，其抑制类型与比生长速率相似。蛋白质产量表明，培养物在约50% RW时受氮限制。指数生长期蛋白质合成的最大比速率在50% RW时为3.7 mg/gh。研究发现，生物质脂质部分的组成取决于用作营养培养基的废水百分比，在含100% RW的培养物中，单不饱和脂肪酸、多不饱和脂肪酸和必需脂肪酸的百分比达到最高。
