Beijing University of Chemical Technology, Beijing, China 10029.
Mol Biotechnol. 2007 Feb;35(2):119-27. doi: 10.1007/BF02686106.
Xanthomonas oryzae pv. oryzae is the pathogen that causes bacterial leaf blight in rice. Bacterial leaf blight is the main cause for severe rice underproduction in many countries. However, with conventional methods it is difficult to quickly and reliably distinguish this pathogen from other closely related pathogenic bacteria, especially X. oryzae pv. oryzicola, the causal organism of bacterial leaf streak in rice. We have developed a novel and highly sensitive real-time method for the identification of this specific bacteria based on a TaqMan probe. This probe is designed to recognize the sequence of a putative siderophore receptor gene cds specific to X. oryzae pv. oryzae, and can be identified from either a bacterial culture or naturally infected rice seeds and leaves in only 2 h. The sensitivity of the method is 100 times higher than that of the current polymerase chain reaction (PCR) gel electrophoresis method for diagnosis.
稻黄单胞菌（Xanthomonas oryzae pv. oryzae）是引起水稻细菌性条斑病的病原菌。细菌性条斑病是许多国家水稻减产的主要原因。然而，用常规方法很难快速可靠地将这种病原菌与其他密切相关的病原菌区分开来，尤其是稻黄单胞菌（X. oryzae pv. oryzicola），它是水稻细菌性叶枯病的病原体。我们已经开发出一种基于 TaqMan 探针的新型、高灵敏度的鉴定该特定细菌的实时方法。该探针设计用于识别稻黄单胞菌 pv. oryzae 特定的铁载体受体基因 cds 的序列，并且可以在 2 小时内从细菌培养物或自然感染的水稻种子和叶片中识别出来。该方法的灵敏度比目前用于诊断的聚合酶链反应（PCR）凝胶电泳方法高 100 倍。
