Faculty of Sciences, University Saint-Joseph, Beirut, Lebanon.
Mol Biotechnol. 2007 Feb;35(2):171-7. doi: 10.1007/BF02686112.
gyrB DNA fragments of seven Bacillus thuringiensis local collection family representatives were amplified by PCR and sequenced. Several differences in their corresponding sequences were evidenced. Both in silico and in vitro restriction maps of gyrB sequences and fragments respectively confirmed that EcoRI and Sau3AI could be used to differentiate between B. thuringiensis strains. However, the phylogeny analysis showed that only the gyrB PCR-Sau3AI allows a strains classification that correlates very well with that obtained on the basis of the sequences analysis. Thus, these finds show that gyrB PCR- Sau3AI digestion could be considered as an efficient, rapid, and easy method to make a distinction, not only between strains belonging to the Bacillus cereus group, but also between those belonging to B. thuringiensis.
通过 PCR 扩增和测序，对 7 种苏云金芽孢杆菌地方采集株代表的 gyrB DNA 片段进行了研究。结果表明，它们的相应序列存在一些差异。gyrB 序列及其片段的计算机模拟和体外酶切图谱均证实，EcoRI 和 Sau3AI 可用于区分苏云金芽孢杆菌菌株。然而，系统发育分析表明，只有 gyrB PCR-Sau3AI 允许对菌株进行分类，该分类与基于序列分析获得的分类非常吻合。因此，这些发现表明，gyrB PCR-Sau3AI 消化可被视为一种有效、快速且简便的方法，不仅可区分属于蜡状芽孢杆菌群的菌株，还可区分属于苏云金芽孢杆菌的菌株。
