Bik Wojciech, Wolinska-Witort Ewa, Baranowska-Bik Agnieszka, Martynska Lidia, Chmielowska Magdalena, Baranowska Boguslawa
Neuroendocrinology Department, Medical Centre of Postgraduate Education, Warsaw, Poland.
Neuro Endocrinol Lett. 2007 Apr;28(2):166-9.
Pituitary adenylate cyclase activating peptide (PACAP 38) is a neuropeptide with anti-inflammatory activity. Vasoactive intestinal peptide (VIP)/PACAP receptors are found in immune cells, endocrine glands and also in adipose tissue. Adiponectin is an adipocyte-derived protein hormone which possesses anti-inflammatory, antidiabetic and antiatherogenic properties. The aim of this study was to examine the influence of PACAP 38 on adiponectin release in basal conditions and during lipopolysaccharide (LPS)-induced acute inflammation.
Male Wistar-Kyoto rats were divided into four groups which received intraperitoneal injections of 0.9% NaCl, LPS, PACAP 38 or LPS+PACAP 38, respectively. Serum adiponectin concentrations were measured using an ELISA test.
LPS administration did not change adiponectin concentration; however, PACAP 38 administered alone decreased serum adiponectin concentration after 2 h (p<0.05) and 4 h (p<0.01). In the group that received LPS+PACAP38, compared with LPS alone, no difference in adiponectin concentration was observed.
We conclude that PACAP 38 may directly modulate adiponectin secretion by adipocytes in basal conditions.
垂体腺苷酸环化酶激活肽（PACAP 38）是一种具有抗炎活性的神经肽。血管活性肠肽（VIP）/PACAP受体存在于免疫细胞、内分泌腺以及脂肪组织中。脂联素是一种由脂肪细胞产生的蛋白质激素，具有抗炎、抗糖尿病和抗动脉粥样硬化特性。本研究旨在探讨PACAP 38在基础条件下以及脂多糖（LPS）诱导的急性炎症过程中对脂联素释放的影响。
将雄性Wistar-Kyoto大鼠分为四组，分别腹腔注射0.9%氯化钠、LPS、PACAP 38或LPS+PACAP 38。采用酶联免疫吸附测定（ELISA）法检测血清脂联素浓度。
给予LPS未改变脂联素浓度；然而，单独给予PACAP 38在2小时（p<0.05）和4小时（p<0.01）后降低了血清脂联素浓度。在接受LPS+PACAP 38的组中，与单独给予LPS相比，未观察到脂联素浓度有差异。
我们得出结论，PACAP 38在基础条件下可能直接调节脂肪细胞的脂联素分泌。
