饮食中的脂肪酸组成对大鼠睾丸线粒体中的视黄醛化反应有不同影响。

Dietary fatty acid composition differently influences retinoylation reaction in rat testes mitochondria.

作者信息

Cione Erika, Senatore Valentina, Tucci Paola, Giudetti Anna M, Genchi Francesco, Gnoni Gabriele V, Genchi Giuseppe

机构信息

Department of Pharmaco-Biology, Laboratory of Biochemistry, University of Calabria, Edificio Polifunzionale, 87036 Rende (CS), Italy.

出版信息

J Bioenerg Biomembr. 2007 Apr;39(2):203-9. doi: 10.1007/s10863-007-9078-x. Epub 2007 Apr 14.

DOI:10.1007/s10863-007-9078-x

PMID:17436146

Abstract

All-trans-retinoic acid (atRA) is incorporated covalently into proteins of rat testes mitochondria. In this study, the effect of three diets with different fatty acid composition on the retinoylation of proteins of rat testes mitochondria has been investigated. Different groups of rats were fed on a basal diet supplemented with 15% of either coconut oil (CO), olive oil (OO) or fish oil (FO). We found that, when compared with CO, the binding of retinoic acid was decreased in FO- and OO-fed rats. Mitochondrial phospholipids composition was differently influenced by dietary treatments; minor changes were observed in fatty acid composition of phospholipids. Few differences were observed in the Arrhenius plots among the three groups of rats. Kinetic analysis revealed a decrease in the V (max) value in FO- and OO- as compared with CO-fed rats. No difference among the three groups were observed in the K ( M ) value. The retinoylation reaction was inhibited by 13-cis-RA and 9-cis-RA.

摘要

全反式维甲酸（atRA）可共价结合到大鼠睾丸线粒体的蛋白质中。在本研究中，研究了三种不同脂肪酸组成的饮食对大鼠睾丸线粒体蛋白质维甲酸化的影响。不同组的大鼠分别喂食添加了15%椰子油（CO）、橄榄油（OO）或鱼油（FO）的基础饮食。我们发现，与喂食CO的大鼠相比，喂食FO和OO的大鼠中维甲酸的结合减少。饮食处理对线粒体磷脂组成有不同影响；磷脂脂肪酸组成有微小变化。三组大鼠的阿累尼乌斯图中观察到的差异很少。动力学分析显示，与喂食CO的大鼠相比，喂食FO和OO的大鼠的V（max）值降低。三组大鼠的K（M）值未观察到差异。13-顺式维甲酸和9-顺式维甲酸抑制维甲酸化反应。