Pawlak Agnieszka, Gil Robert J, Walczak Ewa, Fidziańska Anna
Department of Invasive Cardiology, CSK MWSiA, Warsaw, Poland.
Kardiol Pol. 2007 Mar;65(3):229-35; discussion 236.
Desmin plays one of the key roles in cardiomyocytes. The protein protects the integration of the cell and has the following actions: mechanical, structural and regulatory. Observed abnormalities of its activity have been associated with worsening of heart failure (HF).
Evaluation of desmin activity detected with immunohistochemical (IHC) and immunofluorescent (IF) staining in cardiomyocytes in patients with chronic HF.
The study population comprised 37 patients (mean age 46.5+/-15.28 years, 83.8% males) with diagnosed HF of unknown aetiology, who underwent myocardial biopsy. Coronary angiography was performed to exclude presence of significant coronary artery disease. Heart failure was diagnosed based on clinical assessment and echocardiography showing left ventricular ejection fraction below 45%.
The IHC and IF evaluation of cardiomyocyte desmin showed that these methods were consistent with respect to classification of 31 specimens (83.8%), while being discrepant in 6 (16.2%) cases. Desmin detection in myocardial biopsy specimens with IHC staining showed normal amounts of this protein in 11 (29.8%) cases, excess in 18 (48.6%) patients and deficiency in 8 (21.6%) cases, whereas in IF stained specimens respective values were 12 (32.4%), 15 (40.6%) and 10 (27%). No significant differences were found between all desmin groups (i.e. normal level, excessive and deficiency) evaluated with IHC and IF staining (p=0.39; p=0.25; p=0.31, respectively).
The IHC and IF methods allow evaluation of desmin activity in cardiomyocytes and division into three types of expression. Both methods have high consistency. The IHC, which is the more available method, seems to be a sufficient assay.
结蛋白在心肌细胞中发挥关键作用之一。该蛋白保护细胞的完整性，并具有以下作用：机械作用、结构作用和调节作用。观察到其活性异常与心力衰竭（HF）的恶化有关。
评估慢性HF患者心肌细胞中通过免疫组织化学（IHC）和免疫荧光（IF）染色检测到的结蛋白活性。
研究人群包括37例病因不明的确诊HF患者（平均年龄46.5±15.28岁，83.8%为男性），他们接受了心肌活检。进行冠状动脉造影以排除显著冠状动脉疾病的存在。根据临床评估和超声心动图显示左心室射血分数低于45%来诊断心力衰竭。
对心肌细胞结蛋白的IHC和IF评估表明，这两种方法在31个标本（83.8%）的分类上是一致的，而在6个（16.2%）病例中存在差异。用IHC染色在心肌活检标本中检测结蛋白，11例（29.8%）该蛋白量正常，18例（48.6%）患者过量，8例（21.6%）病例缺乏，而在IF染色标本中相应的值分别为12例（32.4%）、15例（40.6%）和10例（27%）。用IHC和IF染色评估的所有结蛋白组（即正常水平、过量和缺乏）之间未发现显著差异（分别为p = 0.39；p = 0.25；p = 0.31）。
IHC和IF方法可用于评估心肌细胞中的结蛋白活性并分为三种表达类型。两种方法具有高度一致性。IHC是更常用的方法，似乎是一种充分的检测方法。
