Yrjänäinen Heta, Hytönen Jukka, Song Xiao-yu R, Oksi Jarmo, Hartiala Kaija, Viljanen Matti K
Department of Medical Microbiology, University of Turku, Turku, 20520, Finland.
J Infect Dis. 2007 May 15;195(10):1489-96. doi: 10.1086/513873. Epub 2007 Apr 6.
The effect of anti-tumor necrosis factor (TNF)-alpha treatment in Borrelia burgdorferi-infected and ceftriaxone-treated C3H/He mice was evaluated.
Mice were infected with B. garinii A218 or B. burgdorferi sensu stricto N40. At 2 weeks of infection, one group was treated simultaneously with ceftriaxone and anti-TNF-alpha, whereas another received ceftriaxone at 2 weeks and anti-TNF-alpha 4 weeks later. One group received ceftriaxone treatment only. Infected and noninfected control groups were sham treated.
At 14 weeks of infection, B. burgdorferi could not be detected by cultivation or by polymerase chain reaction in tissue samples of any mouse treated with ceftriaxone only. However, spirochetes grew from the tissue samples of one-third of the mice treated with anti-TNF-alpha simultaneously or 4 weeks after ceftriaxone. These activated spirochetes showed ceftriaxone sensitivity rates, plasmid profiles, and virulence rates similar to those of bacteria used to infect the mice. All infected control mice and mice given anti-TNF-alpha only were culture positive.
This report shows that, after ceftriaxone treatment for 5 days, a portion of B. burgdorferi-infected mice still have live spirochetes in their body, which are activated by anti-TNF-alpha treatment.
评估抗肿瘤坏死因子（TNF）-α治疗对感染伯氏疏螺旋体并接受头孢曲松治疗的C3H/He小鼠的影响。
用伽氏疏螺旋体A218或狭义伯氏疏螺旋体N40感染小鼠。感染2周时，一组同时接受头孢曲松和抗TNF-α治疗，而另一组在2周时接受头孢曲松治疗，4周后接受抗TNF-α治疗。一组仅接受头孢曲松治疗。感染组和未感染组作为假处理对照组。
感染14周时，仅接受头孢曲松治疗的任何小鼠的组织样本中，通过培养或聚合酶链反应均未检测到伯氏疏螺旋体。然而，在同时接受抗TNF-α治疗或头孢曲松治疗4周后的小鼠中，三分之一的小鼠组织样本中螺旋体生长。这些活化的螺旋体显示出与用于感染小鼠的细菌相似的头孢曲松敏感率、质粒图谱和毒力率。所有感染对照组小鼠和仅给予抗TNF-α的小鼠培养均呈阳性。
本报告表明，在头孢曲松治疗5天后，一部分感染伯氏疏螺旋体的小鼠体内仍有活的螺旋体，抗TNF-α治疗可激活这些螺旋体。
