Jv Shou-Yong, Ji Fang, Zhu Zi-Min, Yu Zi-Niu, Sun Ming, Tang Zhi-guo, Zhang Rui
College of Life Science and Technology, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
Wei Sheng Wu Xue Bao. 2007 Feb;47(1):88-91.
Parasporal crystal in B. thuringiensis subsp. finitimus T02 forms inside the exosporium and remains attached to the spore after mother cell lysis. Two crystal protein gene cry26Aa and cry28Aa were cloned from this strain. The relationship between the phenomenon of spore-crystal connection and the plasmids was investigated in this work. Mutants BMB1151 curing of the plasmid harboring cry26Aa, and BMB1152 curing of all plasmids were obtained from B. thuringiensis subsp. finitimus T02.The spore-crystal connection didn't appeared when cry26Aa and cry28Aa were transformed back into the strain BMB1152 by shuffle vector alone or in combination together. It suggested the plasmids of B. thuringiensis subsp. finitimus TD2 may contribute to the phenomenon. When gene cry26Aa was transformed back into in the strain BMB1151, spore-crystal connection didn't form either. So the plasmid harboring cry26Aa may also contribute to the phenomenon of spore-crystal connection in B. thuringiensis subsp. finitimus T02.
苏云金芽孢杆菌有限亚种T02中的伴胞晶体在芽孢外壁内形成，在母细胞裂解后仍附着于孢子上。从该菌株中克隆了两个晶体蛋白基因cry26Aa和cry28Aa。本研究对孢子 - 晶体连接现象与质粒之间的关系进行了研究。从苏云金芽孢杆菌有限亚种T02获得了携带cry26Aa的质粒被消除的突变体BMB1151以及所有质粒被消除的突变体BMB1152。当单独或一起通过洗牌载体将cry26Aa和cry28Aa回补到菌株BMB1152中时，未出现孢子 - 晶体连接。这表明苏云金芽孢杆菌有限亚种TD2的质粒可能与该现象有关。当将cry26Aa基因回补到菌株BMB1151中时，也未形成孢子 - 晶体连接。因此，携带cry26Aa的质粒也可能与苏云金芽孢杆菌有限亚种T02中的孢子 - 晶体连接现象有关。
