Severina I S, Piatakova N V, Shchegolev A Iu
Biomed Khim. 2007 Jan-Feb;53(1):44-9.
The influence of polyamines (putrescine, spermidine, spermine) on the activity of human platelet soluble guanylate cyclase and stimulation of the enzyme by sodium nitroprusside, YC-1 and their combination was investigated. All polyamines used stimulated the guanylate cyclase activity and potentiated its activation by sodium nitroprusside. The stimulatory effects of sodium nitroprusside and putrescine (or spermine) were additive; spermidine produced a synergistic activation and increased the additive effect. All polyamines investigated inhibited the activation of the enzyme by YC-1 and decreased the synergistic activation of sodium nitroprusside-stimulated guanylate cyclase activity by YC-1 with approximately the same efficiency. The revealed ability of polyamines investigated to potentiate and synergistically increase (similar to YC-1, but less effective) NO-dependent activation of soluble guanylate cyclase represents a new biochemical effect of these compounds, which should be taken into consideration, especially due to the endogenous nature of polyamines. The data obtained suggest, that the specific functions of polyamines in the processes of cell growth and diffentiation may be also related to the ability of these compounds to activate soluble guanylate cyclase and increase cGMP level.
研究了多胺（腐胺、亚精胺、精胺）对人血小板可溶性鸟苷酸环化酶活性的影响，以及硝普钠、YC-1及其组合对该酶的刺激作用。所有使用的多胺均刺激鸟苷酸环化酶活性，并增强硝普钠对其的激活作用。硝普钠与腐胺（或精胺）的刺激作用具有加和性；亚精胺产生协同激活作用并增强了加和效应。所有研究的多胺均抑制YC-1对该酶的激活作用，并以大致相同的效率降低YC-1对硝普钠刺激的鸟苷酸环化酶活性的协同激活作用。所研究的多胺增强并协同增加（类似于YC-1，但效果较差）可溶性鸟苷酸环化酶的NO依赖性激活的能力代表了这些化合物的一种新的生化效应，应予以考虑，特别是由于多胺的内源性。所获得的数据表明，多胺在细胞生长和分化过程中的特定功能可能也与这些化合物激活可溶性鸟苷酸环化酶并增加cGMP水平的能力有关。
